秀丽小杆线虫耐铜性筛选及其耐铜机制探讨  

Copper on Long-term Role of Nematode Physiological Characteristics

作　　者：宋少娟[1] 郭亚平[1] 阴琨[1] 吴海花[2] 张小民[2] 杨美玲[2] 马恩波[2] 

机构地区：[1]山西大学生命科学与技术学院,太原030062 [2]山西大学应用生物学研究所

出　　处：《四川动物》2008年第5期832-834,共3页Sichuan Journal of Zoology

基　　金：国家自然科学基金30470219;3057024;山西省自然科学基金项目2006011075

摘　　要：为了阐明铜的长期作用对生物体生存状况和生理特性的影响,本文以秀丽小杆线虫Caenorhabditis elegans为材料,在培养基中加入一定浓度的铜(CuSO4.5H2O),最终得到具有Cu2+耐性的线虫(TT),其繁殖率显著降低,丙二醛(MDA)含量显著升高,超氧化物歧化酶(SOD)和谷胱甘肽硫转移酶(GST)比活性显著降低,与重金属络合能力强的柠檬酸含量则显著升高。In order to clarify the long-term effects of copper on organisms living conditions and physical characteristics, the Caenorhabditis elegans was treated by adding a certain concentration of Cu （ CuSO4· 5 H2O） , and at last, the nematode with one strong tolerance to Cu were got. The reproductivity of this kind of nematode was significantly reduced, malonic dialdehyde（MDA） was significantly increased, superoxide dismutase （SOD） and glutathione S-transferase （GST） activity significantly reduced, and citric acid which could chelate heavy metal to complexity was significantly increased.

关 键 词：秀丽小杆线虫 铜 耐受性 生物学特性 生理学特性 

分 类 号：X174[环境科学与工程—环境科学]