脑深部电刺激伏核对吗啡成瘾依赖大鼠学习记忆的影响  

Effect of deep brain stimulation in nucleus accumbens on learning and memory ability of morphine dependence rats

作　　者：王丽丽[1] 王桂松[1] 陈红专 朱亮 陈清山[1] 崔鹏[1] 徐纪文[1] 田鑫[1] 江基尧[1] 罗其中[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,上海200127 [2]基础医学院药理实验室

出　　处：《中国行为医学科学》2008年第9期783-785,共3页Chinese Journal of Behavioral Medical Science

基　　金：上海市科委课题基金项目（06JC14051）

摘　　要：目的探讨脑深部电刺激（DBS）伏核对吗啡成瘾依赖大鼠学习记忆的影响。方法通过DBS电极刺激吗啡成瘾依赖大鼠双侧伏核，于吗啡注射第10天和刺激结束后第1天，进行Morris水迷宫实验比较假刺激组、DBS组、生理盐水组（NS组）大鼠学习记忆能力的变化。结果刺激前3组间的平均潜伏期总体比较差异有显著性（F=7．49，P=0．0077），吗啡依赖组大鼠的平均潜伏期明显长于NS组（P〈0．01）；刺激后3组间的平均潜伏期总体比较差异有显著性（F=7．76，P=0．0069），刺激后DBS组大鼠的平均潜伏期缩短，与假刺激组相比差异具有显著性（P=0．014），较NS组差异无显著性（P=0．43）；假刺激组与NS组相比差异具有显著性（P=0．0056）。刺激前NS组（51．94±3．06）与吗啡依赖组穿越Ⅱ象限时间差异有显著性（P=0．001）；刺激后NS组[（48．53±2．29）s]较假刺激组[（20．74±2．13）Ss]差异有显著性（P=0．001），较DBS组[（38．34±1．68）s]差异无显著性（P=0．062）。结论脑深部电刺激伏核改善了已被吗啡损害的大鼠的学习记忆功能。Objective To explore the effect of deep brain stimulation （DBS）in nucleus accumbens on learning and memory ability of morphine dependence rats. Methods According to stereotaxic atlas of the rat brain, DBS electrodes were implanted in rats bilateral nucleus aceumbens,then Morris water maze test were carried out on the tenth day of morphine injection and the first day after DBS. Results The average time of reaching platform of three groups was different significantly before DBS（F= 7.49, P=0. 0077 ） ;and that of morphine addiction group was longer than saline control group（P 〈 0.01 ）. While after DBS, there was also significantly different （F = 7.76, P = 0. 0069 ） ; the time had significant difference between DBS group and fake stimulation group （ P = 0. 014） ,not saline control group（P = 0.43 ） ;and fake stimulation group compared with saline control group （P = 0. 0056 ）： The time of crossing the platform quadrant（ Ⅱ ）showed significant difference between saline control group and morphine addiction group （P 〈 0. 01 ） ; and the saline control group （48.53 ± 2.29 ）, compared with fake stimulation group （ 20. 74 ± 2.13 ） （P = 0. 001） , DBS group（38.34 ±1.68） （P=0.062）. Conclusion DBS can improve the learning and memory ability impaired by morphine in rats.

关 键 词：脑深部电刺激 伏核 吗啡 MORRIS水迷宫 

分 类 号：R749.64[医药卫生—神经病学与精神病学]