黄荆子乙酸乙酯提取物对人乳癌细胞体内外实验的研究  

Experimentation of the extract from semen viticis negundo with acetoactate on human breast cancer MDA-MB-435S cell in vitro and in vivo

作　　者：尹婵[1] 姜浩[1] 曹建国[2] 陈雪莲[1] 肖集文[1] 

机构地区：[1]南华大学附属第一医院肿瘤内科 [2]南华大学附属第一医院肿瘤研究所

出　　处：《中国现代医药杂志》2008年第9期1-5,共5页Modern Medicine Journal of China

摘　　要：目的研究黄荆子乙酸乙酯提取物(EVn-50)在体内外对人乳癌MDA-MB-435S细胞的抑制作用。方法软琼脂克隆形成法测定细胞生长;溴化尿嘧啶(BrdU)掺入法检测细胞核酸的合成;建立MDA-MB-435S裸鼠异种移植瘤模型,观察经EVn-50处理后的肿瘤抑制率。结果在体外,随EVn-50浓度增高,细胞集落形成率明显下降(P<0.01);EVn-50对MDA-MB-435S细胞核酸合成具有抑制作用,呈剂量依赖性,其中高剂量组的BrdU掺入抑制作用最强,IC50为16.8μg/mL。在体内,EVn-50抑制MDA-MB-435S细胞裸鼠异种移植瘤生长,20、40、80μg/mL的EVn-50对移植瘤的瘤重抑制率分别为18%、31%和63%,呈浓度依赖性。结论EVn-50在体内外对MDA-MB-435S细胞均具有生长抑制作用。Objective To study the inhibition of EVn-50 on human breast cancer cells MDA-MB-435S in vitro and in vivo. Methods Soft agar clone formation assay was carried out to detect the growth inhibitory effect of cells. Nucleic acid synthesis of MDA-MB-435S cells was determined by B rdU incorporate assay.The established of human breast cancer xenografis in nude mouse model was used to test inhibition rate of EVn-50 on human breast cancer. Results In vitro, With concentration higher, The colonyforming rate was reduced drastically （P〈0.01）;EVn-50 significantly inhibited nucleic acid synthesis of MDA-MB-435S cells,in a concentration dependentmanner; and the highest concentration was the most effective, its IC50 was 16.8tzg/mL. In vivo, EVn-50 inhibited the growth of human breast carcinoma xenografts in nude mice. The tumor weight inhibitory rate of EVn-50 at 20,40,80ug/mL was l 8%,31% ,63% respectively, in a dose-dependent manner. The pathological alterations were observed. In EVn-50 groups, degrees of necrosis in tumor tissues were markedly increased respectively compared with the model group. Conclusion EVn-50 can significantly inhibit the growth and proliferation of breast cancer cells MDA-MB-435S in vitro and in vivo.

关 键 词：黄荆子乙酸乙酯提取物 乳腺癌 MDA—MB-4355 移植瘤 抑制作用 

分 类 号：R737.9[医药卫生—肿瘤]