牛瑟氏泰勒虫P23基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of P23 Gene from Theileria sergenti

作　　者：金春梅[1] 张守发[1] 于龙政[1] 

机构地区：[1]延边大学农学院动物医学系,吉林龙井133400

出　　处：《安徽农业科学》2008年第24期10381-10382,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30560112)资助

摘　　要：[目的]为研制牛瑟氏泰勒虫病基因工程苗和诊断试剂盒提供依据。[方法]通过PCR方法从牛瑟氏泰勒虫基因组DNA中克隆了1个P23基因,连接到pGEM-T-Easy载体中。运用生物信息学方法对该基因进行分析。[结果]P23基因全长为684 bp,包含1个长672 bp的开放阅读框,编码223个氨基酸,相对分子量是25.886 kD,等电点pI为9.22,包括1段19个氨基酸组成的信号肽和2段跨膜区。该序列在GenBank上的注册号为EU573168,与瑟氏泰勒虫Chitose型(D84446)和Ikeda型(D84447)的同源性分别为99%、90%。[结论]P23基因编码的蛋白有较好稳定性和免疫原性,可作为制备牛瑟氏泰勒虫基因疫苗的候补抗原。[ Objective] The aim of this study is to provide basis for developing genetic engineering vaceine and diagnostic kit for Theileria sergenti infection. [ Objective] P23 gene of Theileria sergerai was amplified from its genonfic DNA by PCR amplification, and cloned into the pGEM-Easy vector; then the sequencing result was analyzed with bioinformatics methods. [ Result] Whole length of the P23 gene from Theileria sergenti is 684 bp containing a 672 bp open reading frame. The deduced amino acid sequence （223 amino acid residues） contains a signal peptide of 19 araino acid residues and two fragments of transmembrane domains, with relative molecular weight of the 25.886 kD and with the pI of 9.22. The homology between the yielded sequence and Chitese of Theileria sergenti P23 gene（ TS-Chitose type, D84446）, Ikeda of Theileria sergenti P23 gene（TS-Ikeda type, D84447） reached 99% and 90%, respectively. The sequenee has been accessed in GenBank （EU573168）. [Conclusion] The protein encoded by the P23 gene has better stability and immunogenicity, thus can be used as the antigen candidate for preparing genetic engineering vaccine for Theileria sergenti.

关 键 词：牛瑟氏泰勒虫 P23基因 克隆 生物信息学 

分 类 号：S858.23[农业科学—临床兽医学]