动物微生态制剂鸡肠源乳球菌的分离与鉴定  

Isolation and identification of the Lactococcus from the intestines of chicken for Animal Micro-Ecological Agent(AMEA)

作　　者：孟军[1] 刘淑英[1] 万海霞[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021

出　　处：《农业科学研究》2008年第3期30-33,共4页Journal of Agricultural Sciences

基　　金：宁夏大学科研基金资助项目

摘　　要：试验的菌种是从宁夏固原山区完全自然生长的土种健康产蛋母鸡的大肠和盲肠中分离获得的,共分离出44株菌株.通过对这44株菌株的菌落形态观察和革兰氏染色镜检,筛选出12株进行了乳球菌属的生理生化鉴定,初步确定12株菌株属于乳球菌属(Lactococcus).通过糖醇类发酵产酸鉴定,确定8株为植物乳球菌(L.plantarum),4株为棉籽糖乳球菌(L.raffinolactis).各项鉴定结果均符合乳球菌属和相应种鉴定标准.The bacterial species in this experiment were isolated from the large intestine and blind intestine of healthy local hens growing in the total natural environment in the mountain area of Guyuan, Ningxia. 44 strains were isolated. Though the observation of colonial morphology and gram staining, 12 strains were chosen to be identified physiologically and biochemically according to the diagnostic characteristics of Lactococcus and were preliminarily defined as Lactococcus. Through the identification of fermentation and acid-producing of sugar and alcohol, 8 strains were defined as L. plantarurn, 4 strains as L. raffinolactis. All the results were coincidental to the identification criteria of genus and related species of Lactococcus.

关 键 词：乳球菌 生理生化鉴定 微生态制剂 益生菌 

分 类 号：S858.31[农业科学—临床兽医学]