稀土元素Ce对雨生红球藻生长及虾青素积累影响的研究  

Effects of cerium on cell growth and astaxanthin production of Haematococcus pluvialis

作　　者：李哲[1] 蔡明刚[1,2] 黄水英[1] 石荣贵[1] 陆晓霞[1] 齐安翔[1] 吴锐[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005 [2]福建省海洋化学与应用技术重点实验室,福建厦门361005

出　　处：《海洋科学》2008年第9期37-41,共5页Marine Sciences

基　　金：厦门市科技项日(3052Z20031086);新加坡厦门大学校友基金会项目(2007);2007年度大学生创新性实验计划项目(2007);第十一届"挑战杯"全国犬学生课外学术科技作品竞赛项目(2008)

摘　　要：采用反相高效液相色谱分析法(RP-HPLC)研究了稀土元素铈(Ce^(3+))对雨生红球藻(Haematococcuspluvialis)生长及虾青素积累的影响。结果表明,低质量浓度的 Ce^(3+)对微藻生长和虾青素积累均具有明显的促进作用,当 Ce^(3+)的质量浓度为0.1 mg/L 时,对藻生长的促进效果最佳,细胞密度较对照组提高34%;当 Ce^(3+)的质量浓度为1 mg/L时,虾青素质量分数可达到细胞干质量的3.2%,较对照组提高167%。此外,高质量浓度 Ce^(3+)的对雨生红球藻有抑制作用,当 Ce^(3+)的质量浓度高于40 mg/L 时,红球藻的生长完全被抑制,虾青素质量分数也明显降低。The effects of rare earth element cerium on the cell growth and astaxanthin production of Haematococcus pluvialis were studied. The results showed that lower concentration of Ce^3＋ could improve both the growth rate and astaxanthin production of the microalgae. When the Ce^3＋ of 0.1 mg/L concentration was added, the cell number of H. pluvialis was 34% more than the controls. 1 mg/L concentration was the best to accumulate astaxanthin. The astaxanthin content under stress condition could be 3.2% of the cell dry mass, which was 167% higher than the controls. Additionally, higher concentration of Ce^3＋ restrained the growth and astaxanthin production of H. pluvialis.

关 键 词：铈 虾青素 雨生红球藻(Haematococeus pluvialis) 生长 积累 

分 类 号：Q935[生物学—微生物学]