外源脱落酸提高金鱼藻抗铜绿微囊藻胁迫能力的研究  

Effects of Exogenous Abscisic Acid on the Tolerance of Ceratophyllum demersum to Microcystis aeruginosa Stress

作　　者：李小路[1] 潘慧云[1] 徐洁[1] 鲜啟鸣[1] 高士祥[1] 尹大强[2] 邹惠仙[1] 

机构地区：[1]污染控制与资源化国家重点实验室,南京大学环境学院,江苏南京210093 [2]长江水环境教育部重点实验室,同济大学环境科学与工程学院,上海200092

出　　处：《农业环境科学学报》2008年第5期1980-1984,共5页Journal of Agro-Environment Science

基　　金：国家重点基础研究发展规划资助项目(2002CB412307);中国博士后科学基金(20070410338)

摘　　要：以高等沉水植物金鱼藻为试验材料,采用光照培养箱,研究了金鱼藻与铜绿微囊藻共生情况下外施脱落酸对金鱼藻的影响。共生情况下分别采用0、0.5、1.0、2.5、5.0mg·L-1脱落酸进行处理,以金鱼藻单独培养作对照,培养5d后测定金鱼藻生物量及各生理生化指标。结果表明,铜绿微囊藻会对金鱼藻产生胁迫伤害,使其生物量减少。而低浓度(≤1.0mg·L-1)脱落酸处理可以增加金鱼藻光合色素以及可溶性蛋白含量,提高超氧化物歧化酶(SOD)及过氧化物酶(POD)活性,并减少丙二醛(MDA)积累量,减轻金鱼藻所受胁迫。高浓度脱落酸处理使金鱼藻光合色素含量降低,但并不影响其生长。施用0.5￣1.0mg·L-1脱落酸对提高金鱼藻抗胁迫能力的效果最好。Effects of exogenous abseisie aeid（ABA） on the biomass and some physio-biochemieal indices such as chlorophyll, carotenoid, soluble protein, superoxide dismutase（SOD）, peroxidase （POD） and malondialdehyde （MDA） in leaves of Ceratophyllum demersum with or without Microeystis aeruginosa, stress were determined. C. demersum was treated with abseisie acid （0, 0.5, 1.0, 2.5, 5.0 mg·L^-1,respectively） while eo-euhivated with M. aeruginosa, and C. demersum euhivated separately as control test. These physio-bioehemical indices were assayed after five days＇ euhivation. Results showed that M. aeruginosa lessened activities of SOD and POD, facilitated MDA accumulation and led to loss of chlorophyll-a, chlorophyll-b, carotenoid and soluble protein in the leaves of C. demersum. Exogenous ABA of low concentration （≤ 1.0 mg·L^-1） enhanced activities of SOD and POD, reduced MDA accumulation, and increased the content of chlorophyll-a, chlorophyll-b, carotenoid and soluble protein in the leaves of C. demersum, and thus improved the ability of C. demersum to resist M. aeruginosa stress. Exogenous ABA of high concentration decreased the content of chlorophyll and carotenoid, but levels of soluble protein, SOD, POD and MDA were all similar with low concentration ABA treatment, and the growth of C. demersum was not influenced. Exogenous ABA could enhance the resistance of C. demersum against M. aeruginosa stress, and the most suitable concentration is in the range of 0.5-1.0 mg·L^-1.

关 键 词：金鱼藻 脱落酸 铜绿微囊藻 胁迫 

分 类 号：X173[环境科学与工程—环境科学]