重离子辐照对人舌鳞癌Tb细胞周期的影响  

Effect of irradiation with carbon-ion beams on cell cycle after in human squamous cell carcinoma Tb of tough

作　　者：侯玮玮[1] 刘斌[1] 李宁[2] 张洁[1] 张红[2] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000 [2]中国科学院近代物理研究所

出　　处：《山东医药》2008年第24期28-30,共3页Shandong Medical Journal

基　　金：中国博士后基金资助项目(20070410812);甘肃省重大科技专项项目(O702NKDA045)

摘　　要：目的探讨重离子辐照对人舌鳞癌Tb细胞周期进程的影响及受照剂量和修复时间的关系。方法采用流式细胞仪检测不同剂量重离子辐照后人舌鳞癌Tb细胞不同时间点的细胞周期变化。结果人舌鳞癌Tb细胞经重离子辐照后,出现G2/M期明显阻滞,阻滞程度具有剂量和时间依赖性,G2/M期阻滞在24h时与照射剂量呈正相关(r=0.935,P<0.05)。当受到0.5、1Gy照射后,细胞在12h到达阻滞高峰,24h时最大峰值回落。辐照剂量分别为2、4Gy时,细胞表现为G2/M的明显阻滞,未出现阻滞解除现象。结论人舌鳞癌Tb细胞经重离子照射后存活后代生长延缓,放射敏感性增高,呈G2/M期阻滞。Objective To study the cell cycle alterations in cells of human tounge cancer cell line Tb in vitro after carbon-ion irradiation. Method The cell cycle profile were analyzed by flow cytometry. Results After carbon-ion irradiation, there was a positive correlation between irradiation dosage and retardation strength in G2/M phase （ P 〈 0.05 ）. The peak value appeared at 12 h after 0.5,1Gy exposure, and then deceased significantly at 24h. While this release of retardation did not occur after 2.0,4Gy. Conclusion Human squamous cell carcinoma Th of tough is showed significant G2/M arrest after carbon ion irradiation.

关 键 词：重离子辐照 舌鳞状细胞癌 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤] Q274[医药卫生—临床医学]