二硫苏糖醇复合物对SD大鼠硒性白内障的抑制作用  

Comparison the effect of dithiothreitol and its complex on sodium-selenite-induced cataract of SD neonate rats

作　　者：付清[1] 耿宇[3] 张建荣[2] 谢伯林[3] 彭亚军[1] 柳林[1] 李闻捷[2] 

机构地区：[1]第二军医大学长海医院眼科,中国上海市200433 [2]成都军区昆明总医院眼科,中国云南省昆明市650032 [3]第二军医大学长海医院中心实验,中国上海市200433

出　　处：《国际眼科杂志》2008年第9期1767-1769,共3页International Eye Science

基　　金：军队十一五基金(No.06MB213)~~

摘　　要：目的：探讨二硫苏糖醇（dithiothreitol,DTT）及DTT复合物（含阿司匹林（Asp）、还原型谷胱甘肽（GSH）、赖氨酸（Lys）对SD大鼠硒性白内障的抑制作用。方法：对出生后12d的SD乳鼠（约25g）行皮下注射Na2SeO3（0.2mL/次,隔天注射1次,共计3次,累计剂量约210μg）,注射开始后6d（鼠龄18d左右）90%以上的大鼠晶状体发生核性白内障。造模成功后将SD大鼠乳鼠随机分成4组：对照组（C,单独一窝或者是同窝鼠的左眼）、用药组一（D）、用药组二（DAGL）、用药组三（AGL）。分别于造模完成后的第7d给药。C组对照生理盐水点眼;D组25mmol/L的DTT生理盐水溶液点眼;DAGL组DTT复合物（DTT 25mmol/L、Asp 5mmol/L、GSH 2.5mmol/L、Lys 5mmol/L、生理盐水）点眼;AGL组给予不含DTT之外的其他药物成分的生理盐水溶液（Asp 5mmol/L、GSH 2.5mmol/L、Lys 5mmol/L）点眼。5次/d,1滴/次（约25μL）,连续3wk。结果：3d内核性混浊均增大,右眼（用药组）〈左眼（对照组）;3~4d混浊斑块达峰值,大鼠左右眼斑块直径呈现显著差异（P〈0.01）;此后斑块呈密集浓缩趋势,直径均轻度下降;14d混浊斑块密度增加达峰值,左右眼斑块直径仍呈现显著差异（P〈0.01）。20d右眼发展到全白内障（Ⅵ级）的只数〈左眼（P〈0.01）;D、DAGL、AGL组均可抑制SD大鼠硒性白内障的发展,与对照组相比差异显著（P〈0.01）;D、DAGL组对SD大鼠硒性白内障的抑制作用相近（P〉0.05）;D组和DAGL组对SD大鼠硒性白内障的抑制作用均优于AGL组（P〈0.01）。结论：含DTT的抗白内障药物复合物抑制SD大鼠硒性白内障发展的作用,显著强于不含DTT的其它类抗白内障药物组合。AIM- To compare the inhibition effect of dithiothreitol （DTT） and DTT complex （including aspirin （Asp）, glutathione （GSH）, lysine （Lys）） on SD neonate rats sodium-selenite-induced cataract. METHODS： Twelve-day old SD neonate rats （about 25g） were performed subcutaneous injection of Na2SeO3 （0.2mL/time, every other day, totally three times, cumulative dose was about 210μg ）. Six days after the injection began （around 18-day old rat）, more than 90 percent of the lens showed nuclear cataract. SD neonate rats were divided into four groups, the control group （C, selected brood or the left eye in a brood）, DTT treatment group 1 （D）, the treatment group 2 （DAGL）, the treatment group 3 （AGL）. Eye drops were presented 7 days after the SD neonate rats cataract were constructed. Eyes in group C were treated by saline eye drops, group D by 25mmol/L DTT dissolved in saline, group DAGL by D＇I-I- complex （DTT 25mmol/L, Asp 5mmol/L, GSH 2.5mmol/L, Lys 5mmol/L dissolved in saline solution）, and group AGL by dissolution excepted of DTT （Asp 5retool/L, GSH 2. 5mmoVL, Lys 5mmol/L）. The eye drops were instilled into the conjunctiva five times a day,one drop every time （about 25μL）, for 3 consecutive weeks. RESULTS： On the 3rd day, nuclear turbidity increased, the right eye was smaller than the left eye. On the 3-4th day, muddy patch reach peak, the diameter of right eye and the left eye showed significant difference （ P 〈 0.01 ）. There was a plaque-enrichment trend with the turbidity diameter decline mild. On the 14th day, reach density peak and the diameter eye patch still showed significant differences （ P 〈 0. 01 ）. After 20 days, the number of whole cataract in the right eye （ nuclear turbidity level） was less than that in the left eye. The difference had statistical significance （ P 〈 0.01 ）. Compared with control group, inhibition of SD neonate rats cataract was more obvious in D, DAGL, and AGLgroup （P〈0.01）. Similar inhibition eff

关 键 词：SD大鼠 硒性白内障 DTT 

分 类 号：R776.1[医药卫生—眼科] R285.5[医药卫生—临床医学]