TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽粘膜上皮细胞增殖与p16 c-jun基因表达的关系  

Association of Cell Proliferation of Nasal and Nasopharyngeal Epithelium with Expression of p16 and c-jun Genes in TgN (p53mt-LMP1)/HT Transgenic Mice

作　　者：何迎春[1] 田道法[1] 卢芳国[1] 江洁琼[1] 贺安意[1] 

机构地区：[1]湖南中医药大学中西医结合学院,长沙市410007

出　　处：《中国肿瘤临床》2008年第17期1007-1009,1014,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30672738和30572408);湖南省优秀青年基金(编号:03JJY1006)~~

摘　　要：目的:研究TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽黏膜上皮细胞增殖与p16、c-jun基因表达的关系。方法:TgN(p53mt-LMP1)/HT小鼠和野生型C57BL/6J小鼠分别分为诱癌组和对照组,即TgN(p53mt-LMP1)/HT摘要目的:研究TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽黏膜上皮细胞增殖与p16、c-jun基因表达的关诱癌组(TI)、TgN(p53mt-LMP1)/HT对照组(TC)、C57BL/6J诱癌组(CI)和C57BL/6J对照组(CC)4组,H-E染色法观察各组小鼠鼻腔和鼻咽黏膜上皮细胞增殖特征,比较癌前病变率;免疫组织化学染色法检测各组小鼠鼻腔和鼻咽黏膜上皮组织中p16、c-jun基因的表达水平。结果:TI组小鼠鼻腔或鼻咽黏膜上皮细胞非典型性增生明显,TI、TC、CI和CC组小鼠鼻腔和/或鼻咽黏膜上皮癌前病变率分别为90%、10%、0和0。与TC、CI和CC组相比,TI组鼻腔和鼻咽上皮细胞p16基因表达水平显著降低(P<0.01)和c-jun基因表达水平显著增高(P<0.01);与CC组相比,TC组两基因的表达也具有同样的变化趋势(P<0.01)。结论:TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮细胞增殖活性增加,二亚硝基哌嗪可提高TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮细胞的增殖活性,而细胞增殖活性增加与p16基因表达水平下调和c-jun基因表达水平上调密切相关。Objective： To investigate the relationship between the proliferation of nasal and nasopharyngeal epithelium with the expression of p16 and c-jun genes in TgN （p53mt-LMP1）/HT transgenic mice. Methods： TgN （p53mt-LMP1）/HT transgenic mice and the same strain of C57BL/6J wild-type mice of 5 months old were ran- domly divided into two groups： cancer group and the control group. The animals were also divided into 4 groups in total： the TgN （p53mt-LMP1）/HT cancerous lesion-inducing group （TI）, the TgN （p53mt-LMP1）/HT control group （TC）, the C57BL/6J cancerous lesion-inducing group （CI） and the C57BL/6J control group （CC）. At the end of the experiment, the proliferation of nasal mucosa and nasopharyngeal epithelium was evaluated by H＆E staining and the expression of p16 and c-jun genes were determined by imrnunohistochemistry. Resuits： Atypical hyperplasia was significant in nasal and/or nasopharyngeal tissue samples of animals in TI group than in TC, CI and CC groups, with the occurring rates of precancerous lesions of 90%, 10%, 0 and 0, respectively respectively （P〈0.01）. Compared with TC, CI and CC groups, TI group had a lower level of p16 expression and a higher level of c-jun gene expression in nasal and nasopharyngeal epithelium （P〈0.01）. Compared with CC group, TC group had the same trend of p16 and c-jun expression （P〈0.01）. Conclusion： The proliferation of nasal and/or nasopharyngeal epithelium in TgN （p53mt-LMP1）/HT transgenic mice was improved. And N, N＇-dinitrosopiperazine can remarkably promote the proliferation of nasal and/or nasopharyngeal epithelium. The involved mechanisms may be closely related with down-regulation of p16 expression and up-regulation of c-jun expression.

关 键 词：鼻腔 鼻咽 癌前病变 P16 C-JUN 

分 类 号：R739.63[医药卫生—肿瘤]