黄芩有效成分SBM对炎症模型及免疫功能的影响  

Effects of Scutellaria baicalensis fraction on inflammatory animal model and immune function

作　　者：朱伟[1] 孙红光[2] 朱迅[2] 

机构地区：[1]海南大学海洋学院制药工程系,海南海口570228 [2]吉林大学基础医学院免疫学教研室,吉林长春130021

出　　处：《中国药理学通报》2008年第9期1147-1150,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30371334);海南省自然科学基金资助项目(No80413);海南大学自然科学基金项目(Nokyjj0430)

摘　　要：目的研究通过常温超高压方法得到的黄芩有效成分SBM的抗炎作用,并初步探讨其作用机制。方法观察SBM体外对淋巴细胞增殖、IL-1β合成的影响;观察给药后对佐剂性关节炎大鼠原发性及继发性病变、免疫功能及对甲醛诱导足肿胀、醋酸诱导腹腔渗出的影响。结果SBM(31.25～500mg.L-1)体外可明显抑制ConA诱导的小鼠脾淋巴细胞增殖及LPS诱导的腹腔巨噬细胞合成IL-1β;SBM(20mg.kg-1)灌胃给药可明显抑制佐剂性关节炎大鼠原发性及继发性足肿胀,并可抑制脾淋巴细胞增殖、IL-1β合成;SBM(20mg.kg-1)灌胃给药还可明显抑制甲醛诱导的大鼠足肿胀及醋酸诱导的腹腔渗出。结论SBM可通过抑制免疫细胞的功能及促炎因子的产生发挥抗炎作用。Aim To study the anti-inflammatory effects and mechanisms of SBM isolated from Scutellariae radix by high hydrostatic pressure. Methods The effect of SBM on ConA-induced splenocyte proliferation and LPS-induced IL-1β production from peritoneal macrophage was studied in vitro, and the therapeutic effects of SBM on adjuvant-induced arthritis, formalin-induced paw oedema and acetic acid-induced vascular permea- bility were also investigated. Results SBM （ 31.25 - 500 mg · L^-1） significantly inhibited ConA-induced splenocyte proliferation, IL-1β production from perito- neal macrophages in vitro. SBM 20 mg · kg^- 1 inhibited primary and secondary inflammatory reaction, decreased the elevated level of IL-1 β released from peritoneal maerophages, inhibited splenocyte proliferation in adjuvant-induced arthritis in rats. Moreover, SBM inhibited formalin-induced paw oedema and acetic acidinduced vascular permeability. Conclusions SBM could inhibit inflammatory reaction. Its mechanisms might be related to the suppression of immune function and inhibition on proinflammatory cytokines production.

关 键 词：黄芩 炎症 淋巴细胞增殖 IL-Β 

分 类 号：R-332[医药卫生] R284.1