CCK-8对吗啡成瘾大鼠戒断症状及尾核内c-jun蛋白表达的影响  

Effects of CCK-8 on the withdrawal symptoms and expression of c-jun protein in Cd of morphinomania rats

作　　者：杨春晓[1] 杨宝峰[2] 张辉[3] 徐满英[3] 

机构地区：[1]哈尔滨医科大学附属第二医院神经科 [2]哈尔滨医科大学药学院 [3]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150081

出　　处：《中国药理学通报》2008年第9期1166-1170,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30240058);黑龙江省自然科学基金资助项目(NoD9603);黑龙江省科技厅攻关项目(NoGC01C141-01)

摘　　要：目的从行为学、形态学角度,初步研究了八肽胆囊收缩素(CCK-8)对吗啡成瘾大鼠戒断症状及尾核(Cd)内原癌基因c-jun蛋白表达的影响。方法采用动物行为学评估和免疫组化的方法,观察吗啡成瘾大鼠Cd内注入CCK-8(10mg.L-1,1μl)对c-jun蛋白表达和戒断症状的影响。结果①吗啡成瘾组大鼠戒断症状与生理盐水对照组大鼠行为学相比有差异;②吗啡成瘾大鼠在戒断24h时,Cd内注入CCK-8可使戒断症状降低,在戒断40h时戒断症状总分最明显;③单纯吗啡成瘾组大鼠Cd内c-jun蛋白表达与生理盐水对照组大鼠相比下降,而注入CCK-8后c-jun蛋白的表达上升。结论①成功建立吗啡成瘾大鼠模型;②Cd内注入CCK-8可使吗啡成瘾大鼠的戒断症状明显减少;③Cd内注射CCK-8可提高c-jun蛋白的表达。提示CCK-8能调控吗啡成瘾大鼠戒断症状的产生及Cd内c-jun蛋白的表达。Aim To investigate the effects of cholecystokinin octapeptide （ CCK - 8 ） on the withdrawal symp -toms of morphinistic rats by the observation of praxiology,and change of expression of proto-oncogene c-jun in caudate nucleus （Cd） after CCK-8 injected by immunohistochemistry. Methods Animal praxiology evaluation and immunohistochemistry were used. The influence of CCK-8 （10 mg·L^-1,1μl） on the withdrawal symptoms and the expression of c-jun of morphinistic rats was observed. Results vious praxiological changes Morphinistic rats had obin the withdrawal symptoms compared with normal saline rats; the scores of withdrawal symptoms decreased most obviously at withdrawal 40 h after Cd injection of CCK-8 ; the expression of c-jun protein in Cd decreased in morphinistic rats, but increased after Cd injection of CCK-8. Conclusions Models of morphinomania rats were successfully built. The Cd injection of CCK-8 could decrease the withdrawal symptoms of morphinomania rats. The CCK-8 could increase the expression of c-jun protein in Cd. These findings suggested that CCK-8 could regulate the production of withdrawal symptoms and the expression of c-jun protein in Cd of morphinomania rats.

关 键 词：吗啡成瘾 八肽胆囊收缩素 尾核 戒断症状 c-jun 蛋白 

分 类 号：R-332[医药卫生] R322.81