鞘内注射硫酸镁和吗啡对切口痛大鼠脊髓背角p-αCaMK Ⅱ表达的影响  

Effects of intrathecal MgSO_4 and morphine on spinal p-αCaMK Ⅱ in a rat model of incisional pain

作　　者：刘艳阳[1] 胡兴国[2] 龚琴[3] 邹功胜[1] 孔明[1] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室.江苏省麻醉医学研究所,江苏徐州221002 [2]湖南省桃源县人民医院麻醉危重医学科,湖南桃源415700 [3]广东省佛山市中医院,广东佛山528000

出　　处：《中国药理学通报》2008年第9期1208-1212,共5页Chinese Pharmacological Bulletin

基　　金：江苏省级高校重点实验室开放课题资助项目(KJS06002)

摘　　要：目的观察鞘内注射(it)硫酸镁(MgSO4)和吗啡对切口痛大鼠脊髓背角磷酸化钙—钙调蛋白依赖性蛋白激酶Ⅱα(p-αCaMKⅡ)表达的影响。方法所有大鼠术前8d鞘内置管,用机械缩足反射阈值、热缩足潜伏期和累计疼痛评分来评价大鼠疼痛行为学变化;用免疫组织化学和Western blot法来测定吗啡和硫酸镁对大鼠脊髓背角p-αCaMKⅡ表达的影响。结果术前或术后30minit吗啡5μg或MgSO4188μg和吗啡2.5μg使术后2h的机械性缩爪阈值(MWT)和热刺激缩爪阈值(TWL)明显延长(P<0.01),累积疼痛评分明显降低(P<0.01);术前30minitMgSO4375μg或吗啡5μg或MgSO4188μg和吗啡2.5μg使大鼠术后2h脊髓背角p-αCaMKⅡ表达明显减少(P<0.05)。结论在大鼠切口痛模型中,it吗啡5μg或MgSO4188μg和吗啡2.5μg具有确切的抗伤害作用,其抗伤害作用可能与抑制脊髓背角p-αCaMKⅡ表达有关。Aim To observe the effect of intrathecal （it） MgSO4 and morphine on spinal phospho-calcium/ calmodulin-dependent protein kinase Ⅱ α （P-αCaMK Ⅱ） in a rat model of incisional pain. Methods Rats in all groups were intrathecally cathetered on 8 d before operation. Pain behavior at 2 h after incision was evaluated by mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） and a cumulative pain score. The expression of P-αCaMK Ⅱ was as- sessed by immunohistoehemical method and Western blot analysis. Results In pre-ineisional and post-ineisional morphine 5 μg group and pre-incisional and post-incisional MgSO4 188 μg plus morphine 2.5 μg group, MWT and TWL increased significantly and cumulative pain scores decreased remarkably（ P 〈 0. 01 ） 2 h after incision. In pre-incisional MgSO4 375 μg group and pre-incisional morphine 5 μg group and preincisional MgS04 188 μg plus morphine 2. 5 μg group, the expression of P-αCaMK Ⅱ was decreased significantly in spinal cord （P 〈 0. 05 ） 2 h after incision. Conclusions In a rat model of incisional pain, preemptive intrathecal morphine 5 μg and MgSO4 188μg plus morphine 2. 5 μg provided obviously antinociceptire effect, which might be associated with inhibition of the expression of P-αCaMK Ⅱ in spinal cord.

关 键 词：疼痛 硫酸镁 吗啡 钙-钙调蛋白依赖性蛋白激酶Ⅱ 脊髓 

分 类 号：R-332[医药卫生] R322.81