室旁核内微量注射催产素对大鼠胃缺血/再灌注后胃黏膜胃泌素表达的影响  

Effect of microinjection of oxytocin into the paraventricular nucleus on gastrin expression in gastric mucosa following gastric ischemia/reperfusion in rats

作　　者：张咏梅[1] 张文文[1] 张建福[1] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221002

出　　处：《徐州医学院学报》2008年第7期424-428,共5页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30570671);江苏省教育厅自然科学基金(05KJB310134);徐州医学院院课题(07KJ58)

摘　　要：目的研究室旁核(paraventricular nucleus,PVN)内微量注射催产素(oxytocin,OT)对大鼠胃缺血/再灌注(gastric ischemia/reperfusion,GI/R)后胃黏膜细胞胃泌素表达的影响。方法采用夹闭大鼠腹腔动脉30 min,去除动脉夹再灌注1 h的GI/R损伤模型,于单侧PVN微量注射OT。肉眼计数胃黏膜损伤指数,并运用免疫组化实验技术观察了PVN微量注射OT对胃黏膜细胞胃泌素蛋白表达的影响。结果PVN微量注射OT可明显减轻GI/R损伤;PVN内微量注射OT能明显减少GI/R后胃黏膜细胞胃泌素的表达,侧脑室给予OT特异性拮抗剂atosiban后阻断OT对大鼠GI/R损伤的保护作用并使胃泌素的表达增加。结论PVN微量注射OT后对GI/R损伤具有显著的保护作用,这种保护作用在脑内是由OT受体介导的,在胃黏膜局部与抑制胃黏膜细胞胃泌素的表达有关。Objective To observe the effect of microinjection of oxytocin（OT） into paraventricular nucleus（PVN） on gastric ischemia/reperfusion（GI/R） injury and gastrin expression.Methods GI/R injury was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h.A cannula was inserted into the unilateral PVN for microinjection of OT.The gastric mucosal injury index was counted grossly.The expression of gastrin in rat gastric mucosa was examined by immunohistochemistry.Results Microinjection of OT into PVN attenuated the gastric mucosal injury related to GI/R,and significantly decreased the expression of gastrin protein in gastric mucosa.The ptotective effects of OT could be blocked by pretreatment with atosiban,an OT receptor antagonist,into the lateral cerebral ventricle.Conclusion Microinjection of OT into PVN can significantly protect against GI/R injury.These central effects of OT are mediated via its receptors.The local mechanism at the gastric mucosa may involve the inhibition of gastrin expression.

关 键 词：胃缺血/再灌注 室旁核 催产素 胃泌素 

分 类 号：R573.9[医药卫生—消化系统] R338.2[医药卫生—内科学]