高效液相色谱法测定茶叶中多酚  

Determination of Polyphenols from Tea by High Performance Liquid Chromatography

作　　者：孙雯[1] 张铁 董学畅[1] 王森[1] 赵石楠[1] 杨光宇[3] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]文山高等师范专科学校,云南文山663000 [3]云南烟草科学研究院,云南昆明650106

出　　处：《云南化工》2008年第4期29-31,共3页Yunnan Chemical Technology

摘　　要：研究了用高效液相色谱法测定茶叶样品中的6种茶多酚。6种茶多酚用70%甲醇-水溶液提取,以Agilent ZORBAX SB-C18液相色谱柱（4.6 mm×250 mm,5μm）色谱柱为固定相,0.5%醋酸水溶液和甲醇梯度洗脱为流动相,茶叶中主要的茶多酚均达到基线分离;用紫外二极管矩阵检测器检测,得出各组分在其最大吸收波长下的色谱图,根据该色谱图的峰面积定量,并用紫外光谱图对茶叶中主要多酚进行辅助定性。标准回收率为92.5%-102%;RSD为1.2%-1.5%。Determination of 6 polyphenols from tea by high performance liquid chromatography was studied. The poly- phenols was extracted from tea sample by refluxing with 70% methanol, and separated on a Zorbax SB - C18 chromatographic column （4.6 mm× 250 mm, 5μm） eluting with gradient of methanol and 0.5 % acetic acid solution at a flow rate of 1 mL/ min, and monitored by photediode array detector at maximum absorption wavelength. Six polyphcnols from tea sample could be simultaneously determined with recovery of 92.5% - 102% and relative standard deviation of 1.2% - 1.5%.

关 键 词：高效液相色谱 茶叶 多酚 

分 类 号：R284[医药卫生—中药学]