磁性阳离子纳米脂质体的制备及其性能表征  

Preparation and characterization of nano-magnetic cationic liposomes

作　　者：彭健[1] 汤伟[1] 刘路[1] 谭亮[1] 王荣兵[1] 陈伟[1] 周健[1] 

机构地区：[1]中南大学湘雅医院卫生部纳米生物技术重点实验室,湖南长沙410008

出　　处：《中国现代医学杂志》2008年第17期2452-2455,共4页China Journal of Modern Medicine

基　　金：中国博士后科学基金(No:20060390884);国家自然科学基金(No:30672047)

摘　　要：目的探讨磁性阳离子纳米脂质体的制备方法及其性能特征。方法采用薄膜分散法制备磁性阳离子纳米脂质体,并且对其形貌、粒径、磁场响应性、细胞毒性进行表征。结果薄膜分散法制备磁性阳离子纳米脂质体方法简单易行,制备的磁性阳离子纳米脂质体的形貌呈球形,平均粒径为98nm;且具有超顺磁性,在水溶液中的磁场响应性能良好;MTT实验表明磁性阳离子纳米脂质体是毒性较小的基因转染载体。结论薄膜分散法制备的磁性阳离子纳米脂质体粒径小、分布均匀、细胞毒性小,将可作为肝癌基因治疗一种理想的非病毒转染载体。[Objective] To explore preparative method and performance characteristics of nano-magnetie cationic liposomes. [Methods] We prepared nano-magnetic cationic liposomes by film dispersion method .The properties of size, morphology, magnetic field .responsiveness and cell toxicity are studied by some testing means. [Results] The preparative method of nano-magnetie cationic liposomes is very simple and easy. Particle diameter analyzing and TEM indicated that nano-magnetic cationic liposomes present sphericity, the average diameter of particles is about 98 nm. The nano-magnetie cationic liposomes present favorable responsibility to magnetism in water solution and with superparamagnetism. MTr assay showed that nano-magnetic cationic liposomes was a superior gene transfection vector with low toxicity. [Conclusions] Our results showed that nano-magnetie cationic liposomes prepared by film dispersion method were uniform, the diameter and cell toxicity were smaller, It can be used as an ideal non-viral transfection carrier for liver cancer therapy.

关 键 词：磁性阳离子纳米脂质体 肝癌基因治疗 基因转染 表征 

分 类 号：R735.7[医药卫生—肿瘤]