EDSV HN03株纤维蛋白基因的克隆与序列分析  

Cloning and sequence analysis of fiber gene of the egg drop syndrome virus

作　　者：陈红英[1] 李新生[1] 崔保安[1] 夏平安[1] 张红英[1] 廖仲磊[1] 邵攀峰[1] 

机构地区：[1]河南农业大学牧医工程学院

出　　处：《西北农林科技大学学报（自然科学版）》2008年第9期1-4,10,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家“十一五”科技支撑计划专项(2006BAD06A08)

摘　　要：【目的】研究减蛋综合征病毒(EDSV)河南HN03株纤维蛋白基因全序列,为进一步分析EDSV纤维蛋白基因的结构、功能以及EDSV基因工程疫苗的研究奠定基础。【方法】根据GenBank中收录的EDSV纤维蛋白基因序列(Z86065),设计并合成了1对引物,PCR扩增EDSV河南HN03株纤维蛋白基因。将扩增产物克隆入pTG19-T载体并测序。【结果】测序结果表明,HN03株纤维蛋白基因为1935 bp,包含一个完整的开放阅读框,编码644个氨基酸,推导的氨基酸序列有5个潜在的N-糖基化位点,有5个与二硫键形成有关的半胱氨酸。序列分析表明,EDSV HN03株与EDSV国际标准株AV-127纤维蛋白基因核苷酸同源性为99.3%,氨基酸同源性为99.2%;与鹌鹑腺病毒QU株纤维蛋白基因核苷酸同源性为98.9%,氨基酸同源性为98.4%。【结论】腺病毒纤维蛋白基因相当保守。[Objective] The research is tO study the full length nucleic acid sequence of fiber gene of Henan HN03 isolate of egg drop syndrome virus （EDSV）. [Method] One pair of primers was designed and synthesized according to the fiber gene nucleic acid sequence （Z86065） of （EDSV） retrieved from the Gen-Bank. Fiber gene of Henan HN03 isolate of EDSV was amplified by PCR, then was cloned into pTG19-T Easy vector and sequenced. [Result] The result indicated that the full length sequence of fiber gene consisted of 1 935 bp,which included one open-reading frame,encoding 644 amino acid residues. There were five potential N-glycosalation sites and five cysteines in the deduced amino acid sequence. The sequence analysis showed that the identity was 99.3% at nucleotide level between HN03 isolate and EDSV AV-127 strain, and 98. 9% at nucleotide level between HN03 isolate and quail adenovirus QU strain. [Conclusion] It showed fiber gene for adenovirus was highly conserved.

关 键 词：减蛋综合征病毒 纤维蛋白基因 基因克隆 序列分析 

分 类 号：S851.31[农业科学—预防兽医学]