产气法评价纳豆芽孢杆菌对瘤胃液体外发酵的影响  

Assessment of effect on supplementation of Bacillus subtilis natto on ruminal fermentation in vitro by gas production technique

作　　者：邓露芳[1] 王加启[1] 姜艳美[1] 卜登攀[1] 魏宏阳[1] 周凌云[1] 王金枝[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100094

出　　处：《西北农林科技大学学报（自然科学版）》2008年第9期33-39,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：科技部国际科技合作重点项目(2004DFA06700)

摘　　要：【目的】评价纳豆芽孢杆菌对瘤胃液体外发酵的影响。【方法】采用瘤胃液体外发酵产气法,以不添加纳豆芽孢杆菌为对照,动态观测基础日粮中纳豆芽孢杆菌添加量分别为1.5×108/g(处理Ⅰ)和7.5×108/g(处理Ⅱ)对瘤胃液体外发酵产气量、pH值、NH3-N质量浓度和各挥发性脂肪酸浓度的影响。【结果】与对照相比,处理Ⅱ产气量提高了11.89%(P<0.05),瘤胃pH值显著降低(P<0.05),NH3-N质量浓度提高了16.07%(P<0.05),乙酸、丙酸和总挥发性脂肪酸浓度分别提高了10.43%,50.35%和20.70%(P<0.05);处理Ⅰ除丙酸浓度显著高于对照(P<0.05)外,其他各指标均与对照无明显差异(P>0.05)。【结论】纳豆芽孢杆菌培养物在基础日粮中的添加量为7.5×108/g时,能促进瘤胃液对饲料底物的降解代谢。[Objective] The study is to evaluate the role of Bacillus subtilis natto culture （BNC） effect on rurninal fermentation in vitro. [Method] Gas production technique was used to examine the effect of supplementation of 0.15% concentration BNC in dry material （DM） of substrate,containing 1.5×10^8/g （group I）and0.75% concentration BNCin DM of substrate,eontaining 7.5×10^8/g （group Ⅱ） on ruminal gas production, pH value, NH3-N concentration and VFA concentrations in vitro. [Result] Compared with control group （CK）,the gas production in treat group Ⅱ increased by 11.89% （P〈0.05） ,the pH value decreased （P〈0.05） ,the NH3-N concentration increased by 16.07% （P〈0.05） ,and the concentrations of acetic acid, propionic acid and total VFA increased by 10.43% ,50.35% and 20.70% （P〈0.05） respectively. In group I ,propionic acid concentration was higher than that of CK,and the other indicators were not significantly different with CK. [Conclusion] The BNC containing 7.5×10^8/g in DM of substrate promoted the degradation and metabolism of the substrate in ruminal fermentation in vitro.

关 键 词：纳豆芽孢杆菌 产气法 瘤胃液体外发酵 

分 类 号：S816.73[农业科学—饲料科学]