丹芎方对缺氧小鼠脑组织的保护作用  

Protective Effect of Danxiong Recipe on Brain in Hypoxia Mice

作　　者：陈怡[1] 李晓蓉[1] 高建[1] 唐玉[1] 汪明明[1] 薛明[1] 

机构地区：[1]首都医科大学化学生物学与药学院药理学系,北京100069

出　　处：《中药新药与临床药理》2008年第5期366-368,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30472057);北京市科学基金资助项目(7052007);北京市教委科技发展计划项目(KM200410025003)。

摘　　要：目的研究丹芎方对缺氧小鼠脑组织的保护作用。方法取小鼠60只,随机分为正常对照组、模型组、阳性对照组(普萘洛尔组)、丹芎方高、中、低剂量组,连续灌胃7 d后,测定小鼠在常压低氧状态下的存活时间,测定脑组织匀浆的超氧化物歧化酶(SOD)及一氧化氮合酶(NOS)活力,丙二醛(MDA)、乳酸(LD)含量。结果丹芎方可以延长常压低氧状态下小鼠的存活时间,提高SOD活力;降低NOS活力;降低MDA、LD含量。结论丹芎方对缺氧小鼠脑组织具有保护作用。Objective To investigate the protective effect of Danxiong Recipe （DR） on brain in hypoxia mice. Methods Sixty mice were equally randomized to 6 groups： normal group, model group, propranolol group and low - , model - and high - dose DR groups. Except the normal group, the mice in other groups were given the corresponding drug intra - gastrically according to the experimental design, once a day, for 7 days. After treatment, the survival time under nor- mobaric hypoxia condition in different groups was calculated. Meanwhile, the activities of superoxide dismutase（SOD） and nitric oxide synthase （NOS）, and the contents of malondiadehyde （MDA） and lactic acid （LD） in the brain ho- mogenate were detected with test kit. Results The results showed that DR could prolong the survival time of the mice, inhibit obviously the abnormal increase of MDA and LD, reduce NOS activity, and enhance SOD activity in mice brain. Conclusion DR exerts protective effect on brain in hypoxia mice under normal pressure.

关 键 词：丹芎方 超氧化物歧化酶 丙二醛 乳酸 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]