表没食子儿茶素没食子酸酯对乙酸诱导的大鼠结肠炎抗炎作用机制的研究  

Study on Anti-inflammatory Effects and Mechanism of Epigallocatechin-3-gallate in Rats with Colitis Induced by Acetic Acid

作　　者：冉志华[1] 陈迟[1] 萧树东[1] 

机构地区：[1]上海交通大学医学院附属仁济医院上海市消化疾病研究所,200001

出　　处：《胃肠病学》2008年第8期465-468,共4页Chinese Journal of Gastroenterology

基　　金：上海市科委重点实验室项目(06DZ22027)资助

摘　　要：背景:表没食子儿茶素没食子酸酯(EGCG)具有抗氧化作用,能抑制炎症信号通路,如抑制可诱导促炎因子表达的核因子(NF)-κB。目的:探讨EGCG对乙酸诱导的大鼠结肠炎的抗炎作用机制。方法:60只Sprague-Dawley(SD)大鼠随机分为正常对照组(n=10)、模型安慰剂组(n=20)、EGCG治疗组(n=15)和柳氮磺吡啶(SASP)治疗组(n=15)。正常对照组常规饲养;以8%乙酸制备结肠炎模型后,模型安慰剂组、EGCG治疗组和SASP治疗组每日分别予0.9%NaCl溶液2ml、EGCG50mg/kg、SASP0.25g/kg灌胃治疗7d,第8d处死大鼠。酶联免疫吸附测定(ELISA)检测大鼠血清肿瘤坏死因子(TNF)-α和干扰素(IFN)-γ含量,免疫组化SP法测定结肠组织NF-κBp65表达。结果:与模型安慰剂组和SASP治疗组相比,EGCG治疗组大鼠血清TNF-α和IFN-γ以及结肠组织NF-κBp65水平显著降低(P<0.01)。结论:EGCG可通过调节免疫因子表达而达到抗炎的治疗作用,使结肠炎症反应减轻,其疗效优于传统药物SASP。Background： Epigallocatechin-3-gallate （EGCG）,an antioxidant,has been shown to inhibit signaling pathways involved in inflammation,including nuclear factor （NF）-κB,which is an important inducer of proinflammatory factors. Aims： To investigate the anti-inflammatory effects and mechanism of EGCG in rats with colitis induced by acetic acid. Methods：% Sixty Sprague-Dawley （SD） rats were randomly assigned into 4 groups： normal control （n=10）,model placebo （n=20）,EGCG treatment （n=15） and salicylazosulfapyridine （SASP） treatment （n=15） groups. The normal control group was given regular feeding,while the other three groups were given stomach infusion with 0.9% NaCl 2 ml,EGCG 50 mg/kg,SASP 0.25 g/kg daily respectively for 7 days after the establishment of colitis model induced by 8% acetic acid. All rats were sacrificed at the 8th day. The serum levels of tumor necrosis factor （TNF）-α and interferon （IFN）-γ were determined by enzyme-linked immunosorbent assay （ELISA）. The expression of NF-κBp65 in colonic tissue was measured by immunohistochemistry method. Results： Compared with model placebo and SASP treatment groups,the serum levels of TNF-α,IFN-γ,and expression of colonic tissue NF-κBp65 in EGCG treatment group were significantly reduced （P〈0.01）. Conclusions：% EGCG shows its anti-inflammatory effects by modifying the expression of immune factors,thereby ameliorates the colonic inflammatory reaction. Its therapeutic effects are more obvious than conventional drug SASP.

关 键 词：表没食子儿茶素没食子酸酯 结肠炎 肿瘤坏死因子Α 干扰素Ⅱ型 NF-κB 

分 类 号：R285.5[医药卫生—中药学]