重组高效复合干扰素增强宫颈癌CaSki细胞对CTL杀伤作用敏感性及机制的研究  

Study on the Susceptibility of the Cervical Carcinoma Cells to CTL Lysis under the Stimulation of rSIFN-co and Its Mechanism

作　　者：陈妍[1] 何跃东[1] 潘小玲[1] 刘霞[1] 赖曾珍[1] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041

出　　处：《四川大学学报（医学版）》2008年第5期715-718,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技攻关课题(04SG022-010)资助

摘　　要：目的探讨重组高效复合干扰素与同类型复合干扰素、普通重组型干扰素、化疗药物诱导CaSki细胞对CTL杀伤作用敏感性的差异及机制。方法应用重组高效复合干扰素、干复津、干扰素α-2b和顺铂以0.156μg/mL、0.625μg/mL、2.500μg/mL浓度诱导CaSki细胞72h后,用细胞毒性T细胞(CTL)作用于诱导后的CaSki细胞24h,MTT法检测并计算细胞杀伤率;并以流式细胞仪检测CaSki细胞表面CD54、CD40分子表达强度。结果经重组高效复合干扰素诱导后的CaSki细胞对CTL杀伤作用的敏感性高于经另两种干扰素及顺铂诱导的CaSki细胞,这种效应与CaSki细胞表面黏附分子CD54及CD40的表达强度呈正相关,表现出剂量-效应关系。结论重组高效复合干扰素能增加CaSki细胞CD54分子及CD40分子表达强度,从而增加CaSki细胞对特异性效应细胞杀伤作用的敏感性,该作用强于同类型干扰素、普通重组型干扰素及化疗药物。Objective To investigate the susceptibility of cervical carcinoma cells （HPV16^＋） to CTL lysis affected by rSIFN-co, consensus Interferon （Infergen）, IFNα-2b and DDP. Methods After CaSki cervical cancer cells were induced by rSIFN-co, Infergen, IFNα-2b and DDP at the concentration of 0. 156μg/mL, 0. 625 μg/mL, 2. 500 μg/mL for 72 hours, CaSki cells which had been induced were effected by CTL, the cytotoxicity was determined and calculated by MTT assay. The expression intensity of adhesion molecules on Caski cell such as CD54 and CD40 was also determined by flow cytometry. Results The susceptibility of CaSki cell to CTL lysis under the stimulation of rSIFN-co was better than Infergen,IFNα-2b and DDP induced. The expression of CD54 and CD40 on cervical cancer cell was also increased. And this effect had positive correlation to the drug concentration. Conclusion rSIFN-co can increase the expression of CD54 and CD40 on the cervical cancer cell surface, and increase the susceptibility of CaSki cell to specific effective cell lysis in a dose-dependent manner. The effect of rSIFN-co is better than same type interferon, general I type interferon and chemotherapeutic drug induced.

关 键 词：干扰素 宫颈癌 基因重组 

分 类 号：R737.33[医药卫生—肿瘤]