黏液表皮样癌中TSP-1表达与肿瘤血管生成及其生物学行为的关系研究  

Correlation Between the Expression of Thrombospondin-1 and the Angiogenesis in Mucoepidermoid Carcinoma

作　　者：杨森[1] 唐休发[2] 郭丽娟[1] 谭柯[3] 曹玉堂[2] 龚仁国[2] 高庆红[2] 宣鸣[2] 温玉明[2] 王昌美[2] 王晓毅[1] 

机构地区：[1]四川大学口腔疾病国家重点实验室,成都610041 [2]四川大学华西口腔医院头颈肿瘤外科 [3]四川大学公共卫生学院卫生统计学教研室

出　　处：《四川大学学报（医学版）》2008年第5期763-765,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号0040305401042)资助

摘　　要：目的探讨黏液表皮样癌中凝血酶敏感蛋白-1(TSP-1)与肿瘤血管生成及其生物学行为之间的关系。方法采用免疫组织化学SP法检测45例黏液表皮样癌中微血管密度(MVD)值和TSP-1的表达。结果黏液表皮样癌中TSP-1阳性表达率为57.78%(26/45),绝大多数表达于肿瘤细胞的胞浆中,少数表达于肿瘤细胞外基质。在45例黏液表皮样癌组织中平均MVD为(60.68±19.84)/100倍视野,具有TSP-1强表达的肿瘤组织中显示MVD值较低,TSP-1的免疫活性与MVD间呈显著负相关(rs=-0.942,P<0.001)。结论TSP-1在黏液表皮样癌中呈较高表达与黏液表皮样癌中的血管生成呈负相关,可能有抑制黏液表皮样癌中血管生长的作用。Objective To study the relationship between the expression of thrombospondin-1 （TSP-1） and the angiogenesis in mueoepidermoid carcinoma. Methods Immunohistoehemistry were applied to detect the expression of TSP-1 and the value of microvessel density （MVD） in 45 mueoepidermoid carcinoma patients. Results Positive expressions of TSP-1 protein were detected in 26 of the 45 （57. 78%） eases. Most positive staining for TSP-1 was observed in the cytoplasm of the cancer cells, some of those were in the extracellular matrix. The mean MVD in 45 cases with mucoepidermoid carcinoma was 60.68±19.84 vessels per 100 field of vision. Tumors with a high expression of TSP-I showed a low value of MVD and the correlation between TSP-1 immunocompetence and microvessel density was highly significant （rs= -0. 942, P〈0. 001）. Conclusion The TSP-1 is expressed in most mucoepidermoid carcinoma and were associated with neovascularization. TSP-1 is likely to inhibit the extensive neovascularization and increased TSP-1 expression might inhibit angiogenic phenotype in mucoepidermoid carcinoma.

关 键 词：凝血酶敏感蛋白-1(TSP-1) 微血管密度 黏液表皮样癌 

分 类 号：R73-3[医药卫生—肿瘤]