肺腺癌细胞A549 hnRNP B1基因RNA干扰的体外研究  

RNA Interference of hnRNP B_1 Gene in Human A549 Lung Cells

作　　者：蒲丹[1] 周陶友[1] 李为民[1] 吴迪[1] 陈小兵[1] 陈敏[1] 唐凤鸣[1] 韩娟[1] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041

出　　处：《四川大学学报（医学版）》2008年第5期805-807,822,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30270587);教育部2006年博士点专项基金(批准号20060610056)资助

摘　　要：目的体外研究特异的小干扰RNA(siRNA)对肺腺癌细胞A549 hnRNP B1基因表达及生长的抑制作用。方法设计和构建由H1启动子驱动产生的特异的hnRNP B1 siRNA表达质粒,转染至A549细胞,荧光定量PCR法及Western blot检测hnRNP B1基因的表达,MTT法检测该表达质粒转染后对A549细胞生长的影响。结果针对hnRNP B1基因构建的重组质粒具有明显的干扰效果,受特异hnRNP B1 siRNA干扰的A549细胞hnRNP B1 mRNA及蛋白表达下调,细胞生长受到抑制。结论应用RNA干扰技术可阻止hnRNP B1基因的表达,有望成为肿瘤基因治疗新的研究方向。Objective To test the effect of RNA interference of hnRNP BI gene in human lung cell line A549. Methods The RNAs of cell line A549 were interfered by the RNA interference technique. The hnRNP B1 mRNAs were detected by fluorescence quantity RT-PCR. The proteins of hnRNP B1 were detected by Western blot technique. The growth rate of the cells was analysed by MTT. Results The growth of the cells was suppressed significantly. The mRNA and protein expressions of hnRNP B1 were inhibited. Conclusion The expression of hnRNP B1 can be inhibited by RNA interference. RNA interference could become a possible clinical therapy for lung cancers.

关 键 词：肺癌 核内不均一核糖核蛋白B1 SIRNA 

分 类 号：R734.2[医药卫生—肿瘤]