环氧化酶-2基因及蛋白在乳腺癌中的表达及意义  

Expressions of COX-2 gene and protein in breast carcinoma and their significance

作　　者：罗敏[1] 傅春玲[1] 张世琼[1] 刘定荣[1] 

机构地区：[1]涪陵中心医院病理科,重庆涪陵408000

出　　处：《现代医药卫生》2008年第19期2871-2873,共3页Journal of Modern Medicine & Health

摘　　要：目的:探讨环氧化酶-2(cyclooxygenase-2,COX-2)基因及蛋白在乳腺癌中的表达及临床意义。方法:采用原位杂交和S-P免疫组织化学技术检测20例乳腺纤维腺瘤和52例乳腺癌中COX-2mRNA和蛋白的表达。结果:COX-2mRNA和蛋白在乳腺纤维腺瘤和乳腺癌组织中的阳性率分别为(20.0%、65.4%)、(15.0%、57.7%),差异有显著性(P<0.01);COX-2mRNA和蛋白表达之间差异无显著性(P>0.05)。COX-2mRNA表达与淋巴结转移和肿瘤大小有关(P<0.05),与年龄和组织学分级无关(P>0.05);COX-2蛋白表达与淋巴结转移有关(P<0.05),与年龄、肿瘤大小和组织学分级无关(P>0.05)。结论:(1)COX-2mRNA及蛋白在乳腺癌中表达上调,提示COX-2在乳腺癌的发生、发展中起重要作用。(2)COX-2与淋巴结转移正相关,提示COX-2可作为判断乳腺癌预后的标记物。Objective ：To investigate the expressions of COX-2 gene and protein in breast carcinoma and analyze their relationship with clinical factors. Methods：The expression of COX-2 gene and protein were detected on breast fibroadenoma （20 cases） and breast carcinomas （52 cases） by in situ hybridization and immunoshistochemisty （SP method）. Results ：The positive rates of COX-2 mRNA and protein were （20.0%, 65.4%） and （15.0%, 57.7%）in breast carcinoma and breast fibroadenoma respectively.The expression of COX-2 in two groups had statistical difference（P〈0.01）. The expressions of COX-2 mRNA and protein had no statistical difference（P〉0.05）. COX- 2 mRNA expression was related to lymphatic metatasis and tunor size （P〈0.05）,but not to age and histopathological grade （P〉0.05）. COX-2 protein expression was related to lymphatic metatasis （P〈0.05）, but not to age,tunor size and histopathological grade （P〉0.05）. Conclusion： （1）Over-expression of COX-2 may play an important role in the pathogenesis and progression of breast carcinomas. （2） COX-2 expression is related to lymphatic metatasis ,suggesting COX-2 as a molecular marker of breast carcinoma.

关 键 词：乳腺肿瘤 COX-2 原位杂交 免疫组织化学 

分 类 号：R36[医药卫生—病理学]