电针对自由运行状态小鼠SCN内Cry基因表达的影响  

Effect of Electro-acupuncture on Gene Cry Expression in SCN of Free-running Mice

作　　者：周奇志[1] 魏焦禄[1] 蔡定均[1] 张新星[1] 彭晓华[1] 刘旭光[1] 宋开源[1] 赵纪岚[1] 薛红[1] 魏铮[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《四川中医》2008年第9期7-10,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然基金资助(30701125);四川省教育厅自然科学基金资助(2005C006)

摘　　要：目的:研究电针对自由运行状态下小鼠视交叉上核(suprachiasmatic nucleus,SCN)内核心钟基因隐色素基因(cryptochromes gene,Cry)Cry1和Cry2表达的作用,为临床择时针灸效应规律的研究提供分子生物学依据。方法:选用昆明种小鼠,按体重随机分为空白组、捆绑组、电针组。电针组固定动物后,选"百会"和"长强"针刺,电压1-3V,疏密波,频率2/20Hz,时间15min。捆绑组只固定15min,不予其它特殊处理;空白组不予任何特殊处理。在小鼠进入自由运行(free running,FR)状态第3天于近似昼夜时间(circadian time,CT)6:00(即CT6)应用实时荧光定量RT-PCR方法观测电针对SCN内Cry1和Cry2mRNA表达情况的影响。结果:在CT6针刺,有降低Cry1基因表达的作用和下调Cry2基因表达的趋势。结论:针刺导引节律相位的作用可能与其下调SCN内Cry基因的表达、降低负反馈抑制作用有关。Objective： To research the effect of electro- acupuncture on gene Cry expression in suprachiasmatic nucleus （ SCN） of free - running mice, so as to provide evidences of molecular biology for chrono - acupuncture. Methods ： Rats of Kunming species were randomly divided into blank group, binding group and electro - acupuncture group. Applying the method of quantitative RT- PCR, we observed the change of mRNA expression of Cry1 and Cry2 in SCN after acupuncturing ＂Baihui＂ （Du20） and ＂Changqiang＂ （ Du1 ） . Results： The mCry1 mRNA level was decreased and mCry2 mRNA level was tended tobe downregulated by electro- acupuncture in SCN at CT6. Conclusion： The effect of electro - acupuncture is possibly related to the down regulation of genetic expression of mRNA in Cry1 and Cry2 and the reduction of negative feedback inhibition.

关 键 词：电针 昼夜节律 视交叉上核 Cry1基因 Cry2基因 

分 类 号：R245.97[医药卫生—针灸推拿学]