胃癌细胞C-myc蛋白对他莫昔芬的应答  

Response of Protein C-myc to Tamoxifen in Grastric Cancer Cell

作　　者：余怡[1] 曾智[2] 邓昊[3] 

机构地区：[1]江汉大学医学院,武汉430056 [2]天津医科大学病理学系,天津300070 [3]江汉大学肿瘤研究所,武汉430056

出　　处：《江汉大学学报（自然科学版）》2008年第3期73-75,共3页Journal of Jianghan University：Natural Science Edition

基　　金：湖北省自然科学基金(2006AB191);武汉市科技攻关计划引导项目(20055007170);武汉市青年科技晨光计划项目(20045006071-7)

摘　　要：目的:观察C-myc蛋白在胃癌细胞系BGC823中的表达情况,探讨胃癌细胞BGC823经他莫昔芬(TAM)处理后,C-myc蛋白表达的变化.方法:使用10 5MTAM处理胃癌BGC823细胞系24 h后,通过免疫荧光细胞化学的方法及细胞计数,检测C-myc蛋白的表达变化,并通过WST1法检测细胞活力的变化.结果:C-myc在胃癌BGC823中高表达,经10 5 M TAM处理24 h后BGC823细胞活力明显下降(P<0.05,)同时C-myc的表达减弱(P<0.05).结论:C-myc在胃癌细胞BGC823中发挥生物学作用且与TAM介导的BGC823细胞活力下降相关.Objective： To observe the expression of C-myc in gastric cancer BGC823 cell and research the alteration of C-myc expression in gastric cancer BGC823 cell after the treatment of Tamoxifen. Methods： By fluorescence immunocytochemistry staining and cell counting of BGC823 cell treated with 10^-5M Tamoxifen for 24 h, detected the expression alteration of C-myc, at the same time, cell energy was detected by WST-1 method. Results： The over expression of C-myc was detected in gastric cancer BGC823 cell. The decrease of expression of C-myc （P 〈 0.05） and cell energy （P 〈 0.05） were observed in gastric cancer BGC823 cell treated with 10^-5M Tamoxifen for 24 h. Conclusion： C-myc plays an important biological role in the gastric cancer BGC823 cell and is related to cell energy change mediated by Tamoxifen.

关 键 词：C—myc 胃癌 BGC823 他莫昔芬 

分 类 号：R735.2[医药卫生—肿瘤]