自动血细胞分析仪检测外周血造血祖细胞的方法学特点  

Methodological characteristics of automated hematology analyzer in detecting hematopoietic progenitor cells in peripheral blood

作　　者：陈远林[1] 秦立新[1] 张仁生[1] 

机构地区：[1]中南大学湘雅二医院检验科,湖南省长沙市410011

出　　处：《中国组织工程研究与临床康复》2008年第38期7465-7469,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:Sysmex XE-2100就是一种能通过幼稚细胞通道可以检测外周血中造血祖细胞较先进的全自动血细胞分析仪,由于该技术应用时间较短,临床上特别需要了解其检测造血祖细胞的方法学特点、检测性能及临床应用范围。目的:评价全自动血细胞分析仪检测外周血造血祖细胞方法学的特点,并探讨其临床应用。设计、时间及地点:对照观察实验,于2005-05/2006-10在中南大学湘雅二医院完成。对象:异基因外周血造血干细胞移植的健康供者5人,男4人,女1人,年龄20～28岁。急性淋巴白血病患者与非急性淋巴白血病患者各5例,其中男6例,女4例,平均26岁。住院待产孕妇10例,平均27岁。方法:采集动员前后健康供者、白血病患者外周血、采集物及孕妇脐血,采用Sysmex XE-2100全自动血细胞分析仪检测造血祖细胞,同时应用流式细胞仪检测CD34+细胞。主要观察指标:①干细胞移植的健康供者和白血病患者动员后造血祖细胞和CD34+细胞的变化。②待产孕妇分娩时胎儿脐带血中造血祖细胞和CD34+细胞的比较。③高值、中值及低值样品中造血祖细胞检测的批内变异系数CV值。结果:动员后,外周血、采集物与脐带血中造血祖细胞与CD34+细胞均呈良好直线相关性(r>0.77)。高值、中值及低值样品中造血祖细胞检测的批内变异系数CV值均<6.0%。在×106L-1范围内计数造血祖细胞有极好的线性关系(r=0.995)。动员过程中,健康供者于第4天造血祖细胞与CD34+细胞明显上升,于第5天同时达到高峰,但造血祖细胞较CD34+细胞变化幅度更大;白血病患者造血祖细胞与CD34+细胞亦同时上升,同时达到高峰。结论:Sysmex XE-2100全自动血细胞分析仪能快速高效地检测出外周血、脐血和采集物中造血祖细胞,对外周血造血干细胞最佳采集时机的快速判断、干细胞成功采集的预测和白血病患者造血功能恢复的观察等,具有良好的BACKGROUND： Sysmex XE-2100, a fully automated hematology analyzer with an immature information （IMM） channel, can calculate hematopoietic progenitor cells （HPC） in peripheral blood, because of its shortly using, the people want specially to know its characteristics of the technology, function and the rang of using. OBJECTIVE： To develop the characteristics of detecting hematopoietic progenitor cells （HPC） by automated hematology analyzer and to explore its clinical applications. DESIGN, TIME AND SETTING： Controlled trial was performed at the Second Xiangya Hospital, Central South University from May 2005 to October 2006. PARTICIPANTS： Five healthy allogeneic stem cell transplantation donors （4 males and 1 female; aged 20-28 years）, 5 leukemia patients and 5 non-leukemia patients （6 males and 4 females; averagely 26 years in age）, and 10 pregnant women （averagely 27 years in age） were enrolled in this study. METHODS： Peripheral blood from healthy donors, harvest and patients with leukemia, and umbilical cord blood were collected. HPC was calculated by Sysmex XE-2100 fully automated hematology analyzer. CD34^＋ cells were detected by flow cytometry. MAIN OUTCOME MEASURES： HPC and CD34^＋ cells in healthy donors and leukemia patients undergoing stem cell transplantation after mobilization; HPC and CD34^＋ cells in umbilical cord blood; intra lot coefficients of variation of HPC of high, medium and low value. RESULTS： After mobilization, there was a good linear correlation between the HPC and the CD34^＋ cells in peripheral blood, harvest and umbilical cord blood （r 〉 0.77）. The average intra lot coefficients of variation （CV） of HPC of high, medium and low value was 〈 6.0%. The HPC was rather linear （r=-0.995） in the range of × 10^6/L. The HPC and CD34^＋ cells in healthy donors remarkably increased at 4 days of mobilization, and their peak were at 5 days after mobilization, but the HPC was more variable than CD34^＋ cells. The HPC and CD34^＋ cells in ac

关 键 词：血细胞分析仪 造血干/祖细胞 流式细胞术 细胞 CD34^+ 

分 类 号：R394.2[医药卫生—医学遗传学]