Study on NDRG3 in Mesenchyma of Prostate  

作　　者：Wei-qun WANG Yu-hua LI Ai-zhen HONG Biao-yang LIN Xiao-feng JIA Yang LI Run-sheng LI 

机构地区：[1]Department of Pathophysiology, Norman Bethune Medical College, Jilin University, Changchun 130021, China [2]Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China [3]Zhejiang California International NanoSystems Institute, Hangzhou 310029, China

出　　处：《Journal of Reproduction and Contraception》2008年第3期129-136,共8页生殖与避孕（英文版）

基　　金：Shanghai Municipal Committee of Science and Technology (06ZR14072)

摘　　要：Objective To study expression of NDRG3 in prostatic mesenchyma and effect of exogenous NDRG3 on prostatic stromal cells. Methods Immunohistochemical analysis was used to check expression of NDRG3 in prostate mesnenchyma. The WPMY-1 prostate immortalized mesenchyma cell line was stably-transfected with a NDRG3 gene expression vector. The NDRG3-stable transfected WPMY-1 sublines were studied along with parental and empty vector transfected WPMY-1 cells as controls. RT-PCR technology was applied to identity downstream gene expression under regulation of NDRG3 expression.Results Expression of DNRG3 was observed in prostate cancer mesenchyma, over-expression of NDRG3 in WPMY-1 cell up-regulated expression of chemotatic factors-CXCL3 and CXCL5. Conclusion Expression of stromal NDRG3 in prostate cancer specimens is significantly higher than that in benign prostatic hypertrophy （BPH） sample. There is a remarkable difference between the two groups of samples, NDRG3 may be related to angiogenesis in prostatic mesenchyma.Objective To study expression of NDRG3 in prostatic mesenchyma and effect of exogenous NDRG3 on prostatic stromal cells. Methods Immunohistochemical analysis was used to check expression of NDRG3 in prostate mesnenchyma. The WPMY-1 prostate immortalized mesenchyma cell line was stably-transfected with a NDRG3 gene expression vector. The NDRG3-stable transfected WPMY-1 sublines were studied along with parental and empty vector transfected WPMY-1 cells as controls. RT-PCR technology was applied to identity downstream gene expression under regulation of NDRG3 expression.Results Expression of DNRG3 was observed in prostate cancer mesenchyma, over-expression of NDRG3 in WPMY-1 cell up-regulated expression of chemotatic factors-CXCL3 and CXCL5. Conclusion Expression of stromal NDRG3 in prostate cancer specimens is significantly higher than that in benign prostatic hypertrophy （BPH） sample. There is a remarkable difference between the two groups of samples, NDRG3 may be related to angiogenesis in prostatic mesenchyma.

关 键 词：NDRG3 PROSTATE mesenchyma 

分 类 号：R339.21[医药卫生—人体生理学]