富硒蛹虫草提取物硒蛋白多糖对荷瘤小鼠的影响  

Effect of selenium-protein polysaccharide extracted from Se-rich Cordyceps militaris on tumor-bearing mice

作　　者：钟鸣[1] 王丽贺[1] 马慧[1] 郭致富[1] 王成春[1] 刘少霞[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,辽宁沈阳110161

出　　处：《中国中药杂志》2008年第18期2120-2123,共4页China Journal of Chinese Materia Medica

基　　金：辽宁省农业生物技术重点实验室专项资金

摘　　要：目的:提取及分析富硒蛹虫草硒蛋白多糖活性成分,初步探讨富硒蛹虫草硒蛋白多糖的抗肿瘤作用。方法:分别应用Folin-酚试剂法(Lowry法)、苯酚-硫酸法和2,3-二氨基萘荧光分光比色法测定蛋白质、多糖及硒含量,建立荷瘤小鼠模型,分为模型组、环磷酰胺组及虫草高、低剂量组(200,100 mg.kg-1),通过对小鼠体重及抑瘤率、外周血淋巴细胞转化率、廓清指数K、吞噬系数α、肝脏系数及脾脏系数、GSH-Px和SOD活力及MDA含量的测定,观察富硒蛹虫草硒蛋白多糖的体内抑瘤作用及对荷瘤小鼠免疫功能的影响。结果:富硒蛹虫草硒蛋白多糖对荷瘤小鼠具有显著的抑瘤作用,抑瘤率高达46.92%;且提高了荷瘤小鼠的免疫功能,抑制了由环磷酰胺造成的荷瘤小鼠肝脏系数和脾脏系数的下降;增强了机体的抗氧化能力。结论:富硒蛹虫草提取物硒蛋白多糖具有良好的抗肿瘤生长作用。Objective： To extract and analysis the active components of Se-protein polysaccharide from Se-rich Cordyceps militaris, and to discuss the anti-tumor effect of Se-protein polysaccharide. Method： Protein, polysaccharides and selenium content were determined by the methods of Folin-phenol reagent （lowry）, phenol-sulfate and DAN fluorescence, respectively. Tumor-bearing mouse model was established and divided into the model group, cyclophosphamide group, eordyeeps high and low dosage group （ 200, 100 mg· kg^-1 ）. Then the Se-protein polysaccharideg anti-tumor activity and immune function in vivo were observed by compare with model group in the weight of mice, inhibitory rate, conversion rate of peripheral blood lymphocytes, dissection index K, swallowed factor α, liver and spleen factor coefficient, GSH-Px and SOD activity and the content of MDA. Result： Se-protein polysaccharides from Se-rich Cordyceps militaris had a significant anti-tumor action with the inhibitory rate 46. 92% and could avoid toxic effect of chemotherapy drug like cyclophosphamide. It also could enhance immune function and body antioxidant capacity by inhibiting the decline of tumor-bearing mouse liver coefficient and spleen coefficient in Tumor-beating mice caused by cyclophosphamide. Conclusion： Se-protein polysaccha- ride, the extraction of Se-rich Cordyceps militaris＇s can inhibit tumor gronth of tumor-beating mouse.

关 键 词：蛹虫草 硒 抗肿瘤 免疫 抗氧化 

分 类 号：R285.5[医药卫生—中药学]