线栓法制备大鼠局灶性脑缺血模型的改进与体会  

Improvement and Experience in Suture Methods for Rat Model of Focal Cerebral Ischemia

作　　者：杨赞章[1] 陈虹[1] 

机构地区：[1]石河子大学药学院

出　　处：《时珍国医国药》2008年第9期2189-2190,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30560171)

摘　　要：目的建立一种操作简便、稳定可靠的局灶性脑缺血模型。方法分别按Zealonga线栓法和改进线栓法制作局灶性大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)模型,并于术后24 h从神经行为学,脑梗塞率、脑梗塞率的相对标准偏差(RSD)和模型成功率4个方面对两种模型进行评价。结果术后存活的动物均出现了神经功能缺陷症状,两组动物的脑梗塞率相比没有显著性差异(P>0.05),但改进组较Zea-longa组的变异度小,模型成功率高(χ2=4.37,P<0.05)。结论改进法简便可靠,模型成功率高,是较理想的MCAO模型制作方法。To establish a simple, stable and reliable model of focal cerebral ischemia. Methods Along with Zealonga method and modified method, the models of middle cerebral artery occlusion were created. Then after 24 h of operation, these two kinds of model were evaluated by four aspects： the neurological behavior, the cerebral infarction ratio, the relative standard deviation （RSD） of cerebral infarction ratio and the achievement ratio of model. Results All the survival rats demonstrated neurological deficit symptom after 24 h of operation. The cerebral infarction ratio between the two groups was not significant. The RSD of cerebral infarction ratio in the modified group was lower than that in the Zea - longa group while the success rate in the modified group were higher than that in the Zealonga group（x^2 = 4.37, P 〈 0.05）. Conclusion The inodified method of focal cerebral ischemia model is easier to carry out and more reliable. And it is an ideal method for model of MCAO with higher success rate.

关 键 词：线栓法 脑缺血 模型 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]