膀胱肿瘤T24／ADM多药耐药细胞株的建立及耐药机制的研究  

Establishment of multidrug resistance cell line of human bladder tumor T24/ADM and the expression analysis of mechanism of multidrug resistance

作　　者：张建斌[1] 刘红耀[2] 刘尚莹[2] 王泽波[1] 张华屏[3] 冯艳华[1] 

机构地区：[1]山西医科大学研究生处,太原030001 [2]山西医科大学第一临床医院泌尿外科 [3]山西医科大学分子生物学实验室

出　　处：《肿瘤研究与临床》2008年第9期588-591,共4页Cancer Research and Clinic

基　　金：基金项目：山西省高校科技研究开发资助项目（20041005）

摘　　要：目的建立人类膀胱肿瘤多药耐药（MDR）细胞株并研究其耐药机制。方法以人类膀胱肿瘤细胞株T24为研究对象，用多柔比星（ADM）浓度梯度递增诱导法，建立人类膀胱肿瘤细胞多药耐药模型（简称T24／ADM）。用MTT法检测肿瘤细胞的MDR特性；RT—PCR检测膀胱肿瘤耐药细胞株MDR基因的表达情况。流式细胞术检测耐药细胞P-糖蛋白（P—gP）的表达。结果T24／ADM对多种化疗药物产生耐药，对ADM的耐药性提高了16-3倍。RT—PCR检测发现T24／ADM中MDR基因和P—gp的表达明显增强。结论T24／ADM细胞具有MDR特性，其耐药性与MDR基因和P—gP的过表达有关。Objective To establish a multidrug resistance cell line of human bladder tumor and study its characteristics and mechanism of muhidrug resistance. Methods Human bladder tumor cell line T24 was induced to multidrug resistance cell line T24/ADM by intermittent administration of high dose ADM. The multidrug resistance to multianticancer agents of the cells was evaluated by MTY assay; the expression of MDR gene was assessed by RT-PCR and P-gp expression was determined by flow cytometry. Results T24/ ADM resisted to many anti-tumor agents, and its IC50 of ADM was 16.3 times higher than that of T24. Significant overexpression of MDR gene and p-gp of the multidrug resistant cells were detected. Conclusion T24/ADM is a human multidrug-resistant cell line, and it＇ s drug resistance relates to the overexpression of MDR gene and p-gp.

关 键 词：膀胱肿瘤 细胞系 肿瘤 多药耐药相关蛋白类 基因MDR 

分 类 号：R737[医药卫生—肿瘤]