聚合酶链式反应微流控生物芯片的数值模拟  

Numerical simulation of continuous flow-through polymerase chain reaction bio-chip

作　　者：姚李英[1] 贾红阳[2] 陈涛[1] 左铁钏[1] 

机构地区：[1]北京工业大学激光工程研究院,北京100022 [2]中国科学院过程工程研究所,北京100190

出　　处：《计算机与应用化学》2008年第9期1083-1086,共4页Computers and Applied Chemistry

基　　金：国家自然科学基金资助项目(50175002);北京市自然科学基金资助项目(3031001)．

摘　　要：微通道内具有一定流速的DNA反应混和物能否达到聚合酶链式反应(PCR)指定的温度PCR微流控芯片研究的关键问题。本文应用有限体元法(FEV)数值模拟该芯片上3个恒温区的直型、弯型、逶迤型三类微通道内,微流体的温度场和速度场。结果表明:对于宽100μm深50μm的微通道,速度在0.002 m/s～0.02 m/s范围内,180°的弯道以及温度场、温度梯度的存在对其速度场分布无影响,微流体仍呈现为层流;微流体大约经过60μm的距离,其温度场达到稳态,其速度场充分发展为层流;采用宽4 mm深2 mm的空气隔热槽能起到隔热的效果。Polymerase chain reaction technology was widely used in gene diagnostic, gnne recognizing, medicine filtration, new medicine invitation and so on. The temperature and velocity were the important factors for the third PCR technology. The numerical simulation of temperature and velocity field for straight, bending and winding type micro-channels in continuous flow-through polymerase chain reaction biochip was done by using FEV method. The results demonstrated ： micro-fluid was still laminar flow to the micro-channel with 100μm width and 50μm depth. It was not influenced by 180° bending micro-channel, temperature field and temperature grads when the velocity was larger than 0. 002 m/s and less than 0. 02 m/s. The thermal and velocity transition length computed using FEV method was about 60 μm. And the gaps with 4 mm width and 2 mm depth could realize thermal insulation.

关 键 词：有限体元法 聚合酶链式反应微流控芯片 数值模拟 

分 类 号：O242.21[理学—计算数学]