构建新生乳鼠脑性瘫痪模型及其稳定性  

Construction and stability of hypoxic-ischemic encephalopathy models in neonatal rats

作　　者：陈刚[1] 李江[1] 刘伟[1] 李建新[2] 赵丽辉[3] 杨小朋[1] 朱沂[2] 孙振柱[3] 

机构地区：[1]新疆维吾尔自治区人民医院神经外科,新疆维吾尔自治区乌鲁木齐市830001 [2]新疆维吾尔自治区人民医院神经内科,新疆维吾尔自治区乌鲁木齐市830001 [3]新疆维吾尔自治区人民医院病理科,新疆维吾尔自治区乌鲁木齐市830001

出　　处：《中国组织工程研究与临床康复》2008年第37期7326-7329,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：GFP标记的神经干细胞移植治疗脑性瘫痪的实验研究新疆自治区科技攻关和重点科技项目(200633128(2);孕鼠腹腔注射脂多糖合并缺氧制作脑瘫的动物模型新疆自治区卫生厅青年科技人才专项科研基金(2007Y26)~~

摘　　要：背景:脑性瘫痪动物模型制备是否成功目前主要通过生物学行为和组织病理学的检验。目的:建立新生幼鼠缺血缺氧性脑性瘫痪模型,并通过行为学、神经电生理、组织病理学检测其模型的稳定性。设计、时间及地点:随机分组设计的动物对照实验,于2006-10/2007-10在新疆自治区人民医院临床医学研究中心完成。材料:出生7d的清洁级健康Wistar幼鼠24只。方法:24只幼鼠按随机数字表法分为2组,对照组和模型组,每组12只。模型组幼鼠结扎右颈总动脉结合体积分数为0.08的氧气、体积分数为0.92的氮气缺氧环境的方法制作幼鼠脑瘫模型。对照组仅切开幼鼠颈部皮肤后缝合皮肤伤口,未置于缺氧环境。主要观察指标:于生后4,8周,采用足印步态重复间距试验、平衡木试验检测鼠的运动功能,进行行为学评价。生后4周,行为学检测后,应用神经电生理检测仪测定鼠的后肢运动诱发电位。出生后4周取鼠脑组织行苏木精-伊红染色观察脑组织病理变化。结果:各时间点模型组实验鼠患侧的足印步态重复间距和平衡木通过时间较对照组长,差异有显著性意义(P<0.05)。生后4周模型组实验鼠后肢运动诱发电位波幅明显低于对照组,差异有显著性意义(P<0.05)。苏木精-伊红染色结果表明患侧脑室周围细胞排列紊乱,局部囊性变和炎性细胞聚积;患侧大脑皮质神经细胞明显减少,神经细胞变性坏死,胶质细胞反应性增生。结论:应用一侧颈总动脉结扎结合缺氧的方法制作幼鼠脑瘫模型稳定、可靠。神经电生理功能检测对脑性瘫痪动物模型的鉴定提供了更客观、定量的指标。BACKGROUND： Whether animal models of cerebral palsy are established successively mainly depends on the biological behaviour and histological detection. OBJECTIVE： To establish a model of hypoxic-ischemic encephalopathy in neonatal rat, and to detect the stability of the model using behaviour, neuroelectrophysiology and histological examination. DESIGN, TIME AND SETTING： Randomized control trials were performed in the Clinical Medicine Research Center of People＇s Hospital of Xinjiang Uygur Autonomous Region from October 2006 to October 2007. MATERIALS： Twenty-four Wistar 7-day-old neonatal rats were used in this study. METHODS： Twenty-four neonatal rats were randomly divided into two groups, control group and model group, with 12 animals in each grouP. Model group： Pups were induced in the pups at postnatal day 7 （P7） by ligature of the right common carotid artery followed by exposure to hypoxia （0.08 volume fraction of 02 and 0.92 volume fraction of N2 ） for 2h. Control group： Pups （P7） were only induced to incise the anterior cervical skin. MAIN OUTCOME MEASURES： At 4 and 8 weeks after delivery, podogram gait repeated interval trial and balancing beam trial were applied to detect the motor function of the mice and evaluate their behavior. At 4 weeks, motion evoked potential of the rats posterior limbs was assayed with neuroelectrophysiological instrument. Hematoxylin-eosin stain was used to observe the histological changes. RESULTS： The gait repeated interval and the time of crossing balancing beam in the model group was significantly longer than that in the control group （P 〈 0.05）. At 4 and 8 weeks after delivery; the wave amplitude of the motion-evoked potential in rats＇posterior limbs was significantly lower in the model group compared with the control group （P 〈 0.05）. Hematoxylin-eosin staining showed that cells around the affected ventricle arranged in disorder, cystis degeneration and inflammatory cells accumulation were observed in local site; nerve cells in

关 键 词：缺血缺氧性脑病 脑瘫 动物模型 运动诱发电位 病理 

分 类 号：R742[医药卫生—神经病学与精神病学]