肠胃清对人结直肠癌耐长春新碱细胞株HCT8／V的逆转作用  

Reversal of multidrug resistance by "Changweiqing" on vincristine-resistant human colorectal cancer cell line HCT8/V

作　　者：邓皖利[1] 许建华[1] 李长龙[2] 孙珏[1] 张勇[1] 范忠泽[1] 

机构地区：[1]上海中医药大学附属普陀医院肿瘤科,上海200062 [2]华东理工大学药学院,上海200237

出　　处：《肿瘤》2008年第9期755-757,762,共4页Tumor

基　　金：国家自然科学基金资助项目(编号:30672683);上海市教委资助项目(编号:05CZ16)

摘　　要：目的:探讨中药复方肠胃清对人结直肠癌耐长春新碱(vincristine,VCR)细胞株HCT8/V多药耐药的逆转作用。方法:采用细胞增殖抑制实验MTT法观察肠胃清药物血清对HCT8/V细胞株多药耐药性的逆转作用,采用高效液相色谱方法检测肠胃清药物血清作用后的耐药细胞内VCR药物浓度的改变。结果:本实验所采用的人结直肠癌耐VCR细胞株HCT8/V的耐VCR倍数为20.24倍,且对5-氟尿嘧啶、顺铂、羟基喜树碱和丝裂霉素这4种结构和作用机制各异的化疗药物有交叉耐药现象,具有多药耐药性,肠胃清能够逆转HCT8/V的耐药现象。高效液相色谱法结果显示,肠胃清药物血清处理HCT8/V48h后,细胞内的VCR浓度明显增高,且呈剂量依赖性。结论:肠胃清药物血清可提高HCT8/V细胞内化疗药物的浓度,有效逆转耐药细胞的多药耐药现象。Objective ： To investigate the reverse effect of traditional Chinese medicine compound Changweiqing （CWQ） mixture on the vincristine （ V）-resistant human colorectal cancer cell line HCT-8/V. Methods： The reverse effect of serum containing CWQ （SC） on multidrug resistance （MDR） of HCT-8 and HCT-8/V cells was evaluated by MTT assay. Intracellular vincristine concentration in HCT-8/V cells was analyzed by high performance liquid chromatography（HPLC） after treatment with CDS. Results：The vincristine （V） -resistant culurectal cancer cell line HCT-8/V, a MDR cell line, exhibited 20.24-fold resistance to vlncristine and cross-resistance to the four chemotherapeutic drugs, 5-FU, DDP, HCPF and MMC, which had different structures and action mechanisms. It indicated that HCT-8/V had characteristics of muhidrug resistance. SC could reverse drug resistance of HCT-8/V cell line. The data of HPLC showed that the intracellular concentration of vincristine in the HCTS/V ceils was increased by 48-h treatment with SC. The effect was dose-dependent. Conclusion：CWQ elevates the intracellular concentration of vincristine and effectively reverses mulitidrug resistance of HCT-8/V cell line.

关 键 词：结直肠肿瘤 抗肿瘤药(中药) 肿瘤细胞 培养的 抗药性 多药 血清 

分 类 号：R730.52[医药卫生—肿瘤]