他莫昔芬抑制胶质瘤细胞系SHG-44增殖及钠通道电流  

Tamoxifen inhibits cell proliferation and currents of sodium channel in glioma cell line SHG-44

作　　者：王帅[1] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,河北石家庄050000

出　　处：《基础医学与临床》2008年第9期969-972,共4页Basic and Clinical Medicine

摘　　要：目的研究他莫昔芬对SHG-44胶质瘤细胞的增殖及其细胞膜上钠通道电流的作用。方法用四唑盐比色试验分析细胞活性,通过流式细胞仪检测细胞增殖和凋亡。以全细胞膜片钳法记录SHG-44细胞的钠通道电流。结果加入他莫昔芬后,SHG-44细胞变老、脱落,细胞总数减少。他莫昔芬组G2/M期细胞较对照组增多,凋亡细胞比例增加。钠通道电流特性为内向电流、快速激活失活。他莫昔芬可剂量依赖性及电压依赖性阻断该电流。在0mV时,8μmol/L他莫昔芬对钠通道电流抑制率为69%。半数抑制浓度(IC50)为5.54μmol/L。结论他莫昔芬可明显阻断SHG-44胶质瘤细胞上的钠通道,这可能是其抑制胶质瘤细胞增殖的机制之一。Objective To explore the effects of tamoxifen on the proliferation of SHG-44 glioma cells and the currents of sodium channel. Methods The cell activity was detected by MTr. The alteration of cellular proliferation and apoptosis were deetected by flow cytometer. Whole-cell patch clamp technique was used to record the Na currents. Results After treatment with tamoxifen,the cells began aging and shedding and cell counting decreased. The cells in G2/M cell cycle were more than that in control and the apoptosis ration increased. Tamoxifen significantly decreased the amplitude of Na currents of SHG-44 cell line. This blocking effeet was dose-dependent and voltagedependent. When the holding potential was 0 mV, 8 p, mol/L tamoxifen could block this currents by 69%. The half inhibition concentration（ICso） was 5.54 umol/L. Conclusion Tamoxifen can inhibit SHG-44 glioma cells proliferation. The inhibion of sodium channel may be one of its mechanisms.

关 键 词：SHG-44胶质瘤细胞 钠通道 膜片钳技术 他莫昔芬 

分 类 号：R739.4[医药卫生—肿瘤]