子宫颈病变中p16^(INK4A)蛋白表达及其与HPV16/18感染的关系  

Relationship between the expression of p16^(INK4A) protein in the differential cervical lesion and HPV16/18 infection

作　　者：王岩[1] 刘晓霞[1] 邓卉[2] 张少华[1] 毕晓芳[1] 陈利平[1] 马秀萍[1] 贾支红 

机构地区：[1]银川市第一人民医院病理科,750001 [2]银川市第一人民医院妇产科,750001

出　　处：《临床与实验病理学杂志》2008年第4期416-418,422,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：宁夏自然科学基金资助项目(NZ07116)

摘　　要：目的探讨p16INK4A蛋白在子宫颈鳞癌(SCC)和子宫颈上皮内肿瘤(CIN)中的表达及其与HPV16/18感染的关系。方法用原位杂交法检测HPV16/18在25例子宫颈癌、45例CIN及10例慢性子宫颈炎中的表达,同时用免疫组化EliVision法检测p16INK4A蛋白的表达。结果(1)与慢性子宫颈炎相比,CINⅡ级、CINⅢ级、浸润癌HPV16/18杂交信号阳性率显著增高(P<0.01);(2)子宫颈鳞癌组织、CINⅠ级、CINⅡ级、Ⅲ级及慢性子宫颈炎标本中p16INK4A蛋白阳性率分别为100.0%、20.0%、46.7%、100.0%和10.0%;(3)在子宫颈鳞癌及CINHPV16/18感染的标本中p16INK4A蛋白表达均是阳性。结论子宫颈鳞癌的形成与HPV感染、p16INK4A蛋白过表达是呈正相关关系,p16INK4A蛋白可能作为子宫颈鳞癌及CIN的标志物,对子宫颈癌筛查和预防有重要意义。Abstract：Propose To investigate the relationship between the expression of p16It^K4A in cervical squamous cell carcinomas （SCC） and cervical intraepithelial neoplasia （CIN） and HPV16/18 infection. Methods In situhybridization （ISH） were used to detect the expression of HPV16/18 in 25 cases of SCC, 45 cases of CIN and 10 cases of chronic cervicitis. And the expression of p16^INK4A were detected by the immunohistochemical EliVision method. Results （ 1 ） The positive rate of HPV hybrid ;signals in CIN Ⅱ, CIN Ⅲ and SCC were all significantly higher than in chronic cervieitis （ P 〈0. 01 ）. （2） Positive expression rates of p16^INK4A protein in cervical squamous carcinoma,CIN Ⅰ , CIN Ⅱ ,CIN Ⅲ and chronic cervicitis were 100.0% ,20.0% ,46.7%,100.0% and 10.0%. （3） p16^INK4A protein positive was found in which HPV16/18 infection of cervical squamous carcinoma and CIN. Conclusions There showed positive correlation between very high expression of p16^INK4A protein and HPV infection in CIN and SCC. p16^INK4A protein can be an indicator in SCC and CIN, Which has very significance in preventing the HPV infection and can work efficiently in SCC.

关 键 词：子宫颈肿瘤 鳞状细胞癌 子宫颈上皮内肿瘤 人乳头状瘤病毒 P16^INK4A蛋白 原位杂交 

分 类 号：R737.33[医药卫生—肿瘤]