前列腺上皮内瘤基底细胞变化的形态学观察  

Morphologic alterations of basal cells in prostatic intraepithelial neoplasia

作　　者：赵红艳[1] 叶明福[1] 王亚丽[1] 汤金梁[1] 陈锐[1] 

机构地区：[1]第三军医大学新桥医院病理科,重庆400037

出　　处：《临床与实验病理学杂志》2008年第4期439-443,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨前列腺上皮内瘤基底细胞的病变特点,为前列腺良恶性上皮病变的诊断及鉴别诊断提供依据。方法收集28例前列腺(高级别)上皮内瘤标本,应用光镜、免疫组化EliVision Plus二步法,选择34βE12、p63、P504S、PSA、PSAP抗体检测,观察前列腺上皮内瘤中基底细胞的缺失情况。结果HE切片中28例前列腺上皮内瘤的基底细胞变化难以确定,但免疫组化显示6例前列腺上皮内瘤病灶周围基底细胞几乎完全缺失,14例基底细胞断续存在,8例病灶周围基底细胞完整。6例基底细胞几乎完全缺失病例P504S阴性。肿瘤细胞P504S阳性4例,均为基底细胞断续存在病例。结论前列腺上皮内瘤中的基底细胞尽管多数较完整存在,但也存在一定比例的缺失,提示在日益增多的前列腺穿刺标本中,基底细胞完整并非是前列腺上皮内瘤诊断的最重要指标。Purpose To investigate the clinico-pathological features of basal cells in prostatic intraepithelial neoplasia which help provide evidence for diagnosis of prostate epithelial lesions. Methods 28 cases of high grade prostatic intraepithelial neoplasia were studied by hemotoxylin and eosin stain, and the expression of 3413E12, 1363, P504S, PSA and PSAP were detected by immunohistochemical techniques of two-step EliVision Plus method. The absence of basal cells in prostatic intraepithelial neoplasia was analysed according to the immunohistochemical staining. Results Of the 28 cases, total absence of basal cells in 6, partial absence of basal cells in 14, and no absence of basal cells in 8 were observed by IHC, which were uncertain on HE-stained sections. 6 cases with total absent basal cells were P504S negative and 4 cases with partial absent basal cells were P504S positive. Conclusions Although basal cells are complete in most prostate epithelial neoplasia, there remains a high incidence of partial absence of basal cells in some cases, which reveals that the intact of basal cells is not a most significant index on diagnosis of prostate intraepithelial neoplasia, and too much relying on this index may lead to misdiagnosis and under-diagnosis.

关 键 词：前列腺肿瘤 上皮内瘤 基底细胞 P504S 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]