细胞增殖标记物微型染色体维持蛋白2在结肠腺瘤和腺癌中的表达差异及意义  

Expression of minichromosome maintenance protein 2 in colonic adenocarcinoma, adenoma and normal colonic mucosa and its clinical significance

作　　者：王永[1] 周总光[1] 夏庆杰[2] 张文燕[3] 李红光[1] 王蓉[1] 

机构地区：[1]四川大学华西医院消化外科及器官微循环研究室,成都610041 [2]四川大学华西医院眼科实验室,成都610041 [3]四川大学华西医院病理科,成都610041

出　　处：《中华胃肠外科杂志》2008年第5期465-468,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：美国纽约中华医学CMB基金资助（CMB96636）

摘　　要：目的探讨细胞增殖标记物MCM2在结肠癌、结肠腺瘤及正常结肠黏膜中的表达差异及在不同临床病理特征的结肠腺瘤中的表达差异。方法应用免疫组织化学（免疫组化）SP法检测MCM2在正常结肠黏膜、结肠腺瘤及结肠癌中的表达部位；应用实时荧光定量聚合酶链反应法检测MCM2mRNA在12例结肠癌、33例结肠腺瘤及5例正常黏膜中表达量的差异并分析其意义．同时用REST—XL软件分析不同临床病理特征的腺瘤之间MCM2的表达差异及其意义。结果MCM2在正常黏膜中仅表达在腺凹底部，而在结肠腺瘤及腺癌组织中均呈全层上皮表达，但两者在MCM2 mRNA水平上的表达量差异有统计学意义（P=0．001）。结肠腺瘤与正常黏膜相比较，MCM2表达上调。但差异无统计学意义（P〉0．05）。不同临床病理特征的结肠腺瘤之间MCM2表达差异无统计学意义（P〉0．05）。结论MCM2在正常黏膜和结肠肿瘤中表达部位不同，且在结肠腺瘤及结肠癌中的表达量差异显著，可能作为早期筛查诊断结肠癌及评估腺瘤突变的指标之一。Objective To investigate the expression differences of minichromosome maintenance 2 （MCM2） mRNA and protein among colon adenocarcinoma, colon adenoma and normal mucosa, and among different clinicopathological types of adenomas. Methods Fifty specimens, including 33 colonic adenomas, 12 colonic adenocarcinomas and 5 normal colonic mucosa were selected. Each specimen was divided into two parts, one for immunohistochemistry and the other for real-time RT-PCR. Expression differences of MCM2 mRNA among the colonic adenocarcinoma, adenoma and normal colonic mucosa were evaluated by REST-XL software. Results The expression of MCM2 was observed in the basal third to half of the colonic crypts in normal mucosa, while throughout the epithelium in the colonic adenocarcinomas and adenomas. However, the expression of MCM2 mRNA in the adenocareinomas was significantly higher than that in the adenomas （P=0.001）. The MCM2 mRNA expression was elevated in the adenoma with villous type, in the conditions of high-grade dysplasia, larger size, sessile morphology and in patients of older ages, but the difference was not significant by REST-XL （P〉0.05）. Conclusion The difference of MCM2 expression between the adenoma and the adenocarcinoma indicates its potential value in the early diagnosis of colonic cancer.

关 键 词：结肠腺瘤 结肠肿瘤 细胞增殖标记物 微型染色体维持蛋白 实时荧光定量聚合酶链反应 

分 类 号：R735.3[医药卫生—肿瘤]