蛞蝓糖蛋白提出多糖对肺腺癌作用的实验研究  

Effects of LE-DZ on lung adenocarcinoma in vitro and in vivo

作　　者：柳娜[1] 殷志[1] 庄英帜[1] 曹建国[2] 肖集文[1] 韩家凯[1] 封萍[1] 

机构地区：[1]南华大学第一附属医院肿瘤内科,湖南衡阳421001 [2]南华大学肿瘤研究所病理科,421001

出　　处：《临床肿瘤学杂志》2008年第9期779-783,共5页Chinese Clinical Oncology

摘　　要：目的:探讨蛞蝓糖蛋白提出多糖(LE-DZ)在体内外对肺腺癌的抑制作用。方法:细胞计数法检测LE-DZ对人肺腺癌A549细胞生长增殖的抑制作用,并绘制细胞生长曲线;平板克隆实验测定细胞集落形成率;建立小鼠Lewis肺癌模型,测皮下移植瘤体积绘制体积生长曲线,测移植瘤重量,计数小鼠肺表面转移结节数,并计算出抑瘤率和肺转移抑制率;光镜和电镜观察肿瘤组织病理变化。结果:在体外,1、10、100μg/ml浓度LE-DZ均能抑制人肺腺癌A549细胞增殖,并呈浓度和时间依赖性;与对照组比较,细胞集落形成率明显下降(P<0.05)。在体内,LE-DZ能够抑制小鼠Lewis肺癌移植瘤生长和自发性肺转移,25、50、100μg/ml浓度LE-DZ瘤重抑制率分别为19.65%、39.81%、50.96%,肺转移抑制率分别为23.08%、43.27%、68.27%,且呈浓度依赖性。病理学观察结果,LE-DZ可引起肿瘤细胞坏死,诱导肿瘤细胞凋亡。结论:LE-DZ在体外能抑制肺腺癌A549细胞增殖,在体内能抑制小鼠Lewis肺癌皮下移植瘤生长和自发性肺转移,并诱导癌细胞凋亡。Objective：To study the effect of Limax glycoprotein-made polysaccharide （LE-DZ） on lung adenocarcinoma in vitro and in vivo. Methods：Human lung adenocarcinoma A549 cells were cultured in vivro. The inhibitory rates of cells growth were determined by cell counting and the cell growth curve was made. Plate clone formation assay was carried out to detect the phenotypes of colony formation. Lewis lung cancer model was established in C57BL/6 mice in vivo. Subcutaneous transplanted tumor volume was measured and used to draw growth curve. The weight of tumors was measured and the metastatic tumor loci on the lung in mice were counted. The inhibition rates of tumor weight and metastatic tumor loci were calculated. The histopathological changes were observed by lightmicroscopy and electronmicroscopy. Results：In vitro, LE-DZ at 1, 10,100μg · mL^- 1 inhibited the growth and proliferation of A549 cells in a dose-dependent manner and a time-dependent manner. The colony-forming rate was reduced drastically compared with control group （ P 〈 0. 05 ）. In vivo, LE-DZ significantly inhibited the xenograft transplanted tumor growth and spontaneous lung metastasis of Lewis lung cancer in mice in a dose-dependent manner （P 〈 0. 05 ）, with growth inhibition rates of 19. 65%, 39. 81% and 50. 96% ; and metastatic tumor foci rates of 23.08% , 43.27% and 68. 27% , respectively. The pathological alterations were observed. In LE-DZ groups, degrees of necrosis in tumor tissues were markedly increased comparing with the model group and cell apoptosis can be observed. Conclusion ： LE-DZ can inhibit the growth and proliferation of lung adenocarcinoma A549 cells in vitro, and inhibit the growth of Lewis lung cancer transplanted tumor and spontaneous lung metastasis in mice, and induce apoptosis in vivo.

关 键 词：蛞蝓 蛞蝓糖蛋白提出多糖 肺腺癌 移植瘤 

分 类 号：R730.53[医药卫生—肿瘤] R734.2[医药卫生—临床医学]