局部亚低温对大鼠局灶性脑缺血-再灌注模型NKCC1 mRNA水平的影响  

Effects of Local Mild Hypothermia on the mRNA Level of NKCC1 in Focal Brain Ischemia-Reperfusion Injury Rats

作　　者：孙伟[1] 苏志强[1] 刘建丰[1] 盛利[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,黑龙江哈尔滨150001

出　　处：《中国医学物理学杂志》2008年第5期825-827,共3页Chinese Journal of Medical Physics

摘　　要：目的:观察局部亚低温对大鼠局灶性脑缺血-再灌注模型NKCC1 mRNA表达水平的影响,探索局部亚低温的脑保护机制。方法:雄性Wistar大鼠50只,随机分成常温组和亚低温组。应用"线栓法"实现大鼠右侧大脑中动脉阻塞,2 h后拔出线栓进行再灌注,于再灌注3 h、12 h、24 h和72 h处死大鼠。应用RT-PCR方法观察NKCC1 mRNA水平的变化。结果:与假手术亚组相比,缺血-再灌注亚组大鼠缺血区皮质NKCC1 mRNA水平明显升高,开始于再灌注3 h,12 h～24 h达到高峰,72 h开始下降,亚低温组各再灌注时间点NKCC1 mRNA水平均低于常温组中相应亚组的水平。结论:局部亚低温通过抑制缺血-再灌注过程中NKCC1 mRNA水平的上调发挥脑保护作用。Objective： To observe the effect of local mild hypothermia on the mRNA level of NKCC1 in rats with focal brain isehemia-reperfusion and clarify the mechanism of local mild hypothermia in cerebral protection. Methods： Male Wistar rats were randomly divided into normothermia group and hypothermia group. The model of focal brain ischemia-reperfusion injury was induced by middle cerebral artery occlusion （MCAO）. After 2 hours of ischemia and 3, 12, 24 or 72 hours of reperfusion, the animals were sacrificed. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the level of NKCCI mRNA. Results： Compared with sham-operated deuto-group, ischemia-reperfusion significantly increased the expression of NKCC1 mRNA in the ischemic cortex which began at 3 hours after reperfusion,peaked at 12 to 24 hours and subsided at 72 hours. At 3 hours, 12 hours, 24 hours and 72 hours after reperfusion, the level of NKCC1 rnRNA in local mild hypothermia group was much lower than that in normotherrnia group. Conclusions： This study indicates that local mild hypothermia may decrease the level of NKCC1 mRNA during the course of focal brain ischemia-reperfusion and thus become an effective method for cerebral protection.

关 键 词：局部亚低温 脑缺血-再灌注 NKCC1 

分 类 号：R741.05[医药卫生—神经病学与精神病学]