全复配食用菌提取物对C26荷瘤小鼠的抑瘤作用和免疫功能影响  

Anti-tumor and immunoenhancement effects of effective combination of extraction from edible fungi on C26 xenograft mice

作　　者：王琪[1] 李晓昱[2] 文达[1] 孙颖[2] 莫非[2] 朱明洁[2] 

机构地区：[1]四川大学华西药学院研究室,四川成都610041 [2]上海市肿瘤研究所研究室,上海200032

出　　处：《中国癌症杂志》2008年第9期681-684,共4页China Oncology

摘　　要：背景与目的:食用真菌在医疗保健领域的研究展现出强大的应用潜力。该课题探讨全复配食用菌提取物的抗肿瘤作用及对荷瘤小鼠免疫功能的影响。方法:小鼠随机编号分组,其中预防组预先给予全复配食用菌提取物灌胃2周,每天1次,之后各组均接种C26细胞建立荷瘤小鼠模型,接种后第2天给小鼠予以不同制剂灌胃(空白组灌0.9%氯化钠溶液,治疗组、预防阻灌复配食用菌提取物,CTX阳性组腹腔注射CTX)每天1次,观察记录小鼠一般状态,2周后处死小鼠无菌取脾脏、取瘤称重。MTT法检测小鼠脾淋巴细胞转化功能,乳酸脱氢酶(LDH)法检测NK细胞杀伤活性。结果:复配食用菌提取物预防组对小鼠C26肿瘤有30.4%的抑制率;小鼠脾淋巴细胞在ConA刺激下的增殖能力和NK细胞杀伤功能均显著高于空白组。结论:复配食用菌提取物预防性摄入对再接种C26小鼠有抑瘤和免疫增强作用。Background and purpose： Edible fungi has shown powerful potential in health care. This study aimed to explore the anti-tumor and immunoenhancement effects of combination of extraction from edible fungi. Methods： C26 cells has been xenografted into mice, and the animal models were randomly divided into control group, prevention group, treatment group and cyclophosphamide group. Mice were given the combination of extraction from edible fungi every day after vaccination of C26 cells into mice, except the prevention group which has been given the combination of extraction from edible fungi two weeks before vaccination. Two weeks after being planted, all the mice were killed and the tumor inhibition rates were studied. The immune function was measured by T lymphocyte transforming assay and NK killing assay. Results： There were significant difference in terms of tumor weight between prevention group and control group. The prevention group mice display improved T lymphocyte transforming assay and NK killing ability. Conclusions.. The combination of extraction from edible fungi has remarkable inhibitory effects on C26 carcinoma in mice and can enhance immunity against mice with C26 carcinoma.

关 键 词：复配食用菌提取物 抑瘤作用: 免疫增强作用 

分 类 号：R730.1[医药卫生—肿瘤]