便携式光学表面等离子共振生物传感系统设计及试验  

Design and Experiment of a Portable Optical Surface Plasmon Resonance Biosensor System

作　　者：胡敬芳[1] 罗富坤[2] 蒋国良[1] 李振峰[1] 胡建东[1] 赵向阳[1] 赵媛媛[1] 江敏[1] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南职业技术学院,河南郑州450046

出　　处：《河南农业大学学报》2008年第4期410-413,共4页Journal of Henan Agricultural University

基　　金：河南省自然科学基金项目(061103900)

摘　　要：设计了一个用于便携式光学表面等离子共振(SPR)生物传感系统,它由光学SPR生物传感器、微流池、半导体致冷器(TEC)和压紧部件组成.温控微流池温度控制采用PID算法,流动样品与封闭恒温系统进行热交换,实现流动样品与光学生物传感器温度保持恒定.试验表明,当设定温度25℃的最大A/D转换输出值为16 384,温控系统启动4 min后,最大波动量少于±160,实现SPR生物传感器控温精度达±0.01℃,满足实际便携式光学生物传感器应用.A portable optical surface plasmon resonance （SPR） biosensor with a high precision and temperature-controlled was designed, which comprises a portable SPR biosensor, micro-fluidic cell, thermoelectric cooler （TEC） and compacting block. The system, using PID temperature-controlled algorithm, and with thermal exchange going on between flowing sample and closing controlled system for constant temperature, actualizes the be tween the liqucal sample and the optical sple biosensor. The experimental results show that an integer maximum value of 16 384 was obtained from A/D converter when the temperature in the internal cavity corresponded to 25 ℃. However, was the integer value of 160 was obtained from A/D converter by changing the room temperature from 20 ℃ to 35 ℃ after starting the temperature-controlled system for 4 minutes. So the temperature of the sample solution flowed through the surface of the SPR biosensor changed between ± 0.01 ℃ , which meets the needs of the portable optical SPR biosensor.

关 键 词：光学SPR生物传感器 恒温控制器 PID控制 微流池 

分 类 号：TB133[机械工程—光学工程]