分化诱导剂对Eca-109细胞DNA聚合酶β及X-射线修复交联-补充蛋白1表达的影响  

Differentiation effecs on DNA polymerase beta and X-ray repair cross-complementing protein 1 expression in Eca- 109 cells

作　　者：李士坤 徐俊 陈克河 任庆梅 

机构地区：[1]信阳市中心医院消化内科,河南省信阳464000 [2]河南省固始县第二人民医院普外科

出　　处：《中国基层医药》2008年第8期1267-1268,共2页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨恶性细胞在逆转化过程中DNA聚合酶β及x-射线修复交联-补充蛋白1（XRCC1）表达的变化。方法将Eca-109细胞分为诱导组（加β-溴-cAMP）和对照组。两组同等条件下培养48h，用免疫细胞化学的方法检测DNA聚合酶β及XRCC1的表达。结果与对照组相比，诱导组DNA聚合酶β与XRCC1的表达均减弱（P〈0．05）。结论分化诱导剂能下调DNA聚合酶β及XRCC1的表达，高表达的DNA聚合酶β及XRCCt可能是细胞恶性化的表现。Objective To study the change of DNA polymerase beta and XRCC1＇s expression during malignant cell differentiation. Methods The Eca-109 cells were divided into 2 groups： differentiation group which cultured with β-Br-cAMP and control group. The 2 groups cells were cultured 48h simultaneously. The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1. Results Compared with control group, the expression of DNA polymerase beta and XRCC1 was decreased simultaneously（ P 〈 0.05 ）. Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1, suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.

关 键 词：DNA聚合酶Β ECA-109细胞 X-射线修复交联-补充蛋白1 

分 类 号：R73[医药卫生—肿瘤]