枯草杆菌在淀粉液态培养基中生产α-淀粉酶  

Wilted Hay Bacilli Producing α-Amylase in Amylum Culture Medium

作　　者：张程 袁宏志 

机构地区：[1]内蒙古通辽市产品质量计量检测所

出　　处：《光谱实验室》2008年第5期970-973,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家科技攻关计划子课题(批准号:2003BA614A-10-01)资助

摘　　要：以枯草杆菌(Bacillus subtilis BF7658)为实验菌株,以淀粉为主要原料,采用液态培养基发酵,生产α-淀粉酶。结果表明:在23℃至44℃内,培养基起始pH值为自然pH值,产酶最佳培养温度为37℃;枯草杆菌在淀粉液态培养基中控制温度为37℃,振荡培养,其产酶最适宜淀粉与水组成比例为:75∶100;在最佳温度37℃下,淀粉∶水为75∶100时,最佳培养时间为36h。α-amylase was produced based on wilted hay bud spore bacilli as fermentation agent, amylum as main material in the liquid culture medium. The most suitable raise temperature for the enzyme is 37℃ in the range of 23℃ to 44℃, culture medium initialization pH is nature pH. The optimal conditions for producing amylase are in the ratio of amylum ： water= 75 ： 100 by way of vibration cultivate at 37 ℃ for 36h.

关 键 词：Α-淀粉酶 枯草杆菌 淀粉液体培养基 

分 类 号：O652[理学—分析化学] Q55[理学—化学]