光动力对金黄色葡萄球菌的杀伤作用及其AFM观察  

Photodynamic Inactivation of Staphylococcus aureus and Its AFM Images

作　　者：任雅清[1] 唐书泽[1] 金花[2] 吴希阳[1] 毕水莲[1] 陈振强[3] 

机构地区：[1]暨南大学食品科学系 [2]暨南大学化学系,广东广州510632 [3]暨南大学光电工程系,广东广州510632

出　　处：《食品与发酵工业》2008年第8期56-59,共4页Food and Fermentation Industries

基　　金：广州市科技攻关项目(06A1910139);粤港招标项目(2005A20303002)

摘　　要：通过细菌菌落计数以及原子力显微镜图像研究了血啉甲醚对金黄色葡萄球菌的光动力杀伤作用。结果表明:HMME在金黄色葡萄球菌菌液中的最强吸收峰为392nm,溴钨灯光源波长范围350～2500nm,能满足HMME所需的波长;HMME浓度为5μg/mL接受溴钨灯光源光照30min能杀死82.2%的金黄色葡萄球菌;原子力显微镜图像显示其灭菌机制是HMME在光照条件下导致菌体破裂,细胞内容物大量外泄;无光照条件下,HMME对金黄色葡萄球菌杀伤效果不显著。The photodynamic inactivation of Staphylococcus aureus by hematoporyrin monomethyl ether were investigated through the absorption spectrum of HMME in S. aureus suspension, reduction of colony unit and atomic force microscopy images. Results showed that the absorption peak of HMME was 392 nm, the wavelength from 350 nm to 2500 nm of the irradiation source could cater the HMME with the required wavelength; 82.2 % of S. aureus were photoinactivated and most cell membrane was damaged with cell contents leakage from the cell by illumination with 30 min light in the presence of 5 μg/mL HMME; The cell killing effect to S. aureus with illumination was more obvious than in the dark at the same concentration of HMME. S. sureus was very sensitive to the photodynamic inactivation of HMME

关 键 词：血啉甲醚 金黄色葡萄球菌 光动力杀伤作用 AFM图象 

分 类 号：R515[医药卫生—内科学]