使用GeneSifter对小鼠肺钩虫感染基因表达谱的分析  

ANALYSIS OF GENE EXPRESSION PATTERN OF LUNG-STAGE HELMINTH INFECTION IN MICE WITH GENESIFTER

作　　者：刘华[1] 马文丽[1] 柯杰兵[2] 丁大鹏[1] 郑文岭[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]中国人民解放军军事体育进修学院

出　　处：《现代预防医学》2008年第19期3787-3789,共3页Modern Preventive Medicine

基　　金：广东省重点实验室资助(960327)

摘　　要：[目的]从基因水平探索肌萎缩侧索硬化(ALS)其发病机制并发现治疗ALS的新靶点。[方法]通过GeneSifter软件对野生型(WT)和重度联合免疫缺陷型(SCID)小鼠肺组织钩虫感染的微阵列表达谱进行分析,使用two-way ANOVA的统计学方法对数据聚类,并结合Gene Ontology和KEGG(Kyoto Encyclopedia of Genes and Genomes)生物学通路,深入分析具有种系效应的517个差异基因(P﹤0.001)。[结果]显示ALS的信号转导通路在两组小鼠间差异有统计学意义(Z﹥2),与Th2反应相关的多个基因对ALS的代谢异常具有保护作用。[结论]筛选出的3个特异基因,可作为治疗肌萎缩侧索硬化的新候选基因。[Objective] To investigate the molecular pathogenesis of amyotrophic lateral sclerosis {ALS} at genetic level and so as to develop the new targets to cure it. [ Methods ] Methods Used two-way ANOVA, Gene Ontology and KEGG methods for the analysis of gene expression pattern of lung-stage Helminth infection in wild type and SCID mice with GeneSifter software, and then further investigated on 517 genes with strain-effect （P〈 0.001）. [Results] The pathway of amyotrophie lateral sclerosis {ALS} was significantly different between the two type of mice （Z 〉 2）, several genes relevant with Th2 had a positive effect on the abnormal metabolic process of ALS. [Conclusion] 3 novel genes selected to be candidates may have thera- peutic benefits for amyotrophie lateral sclerosis.

关 键 词：GeneSifter软件 基因表达谱 肌萎缩侧索硬化 

分 类 号：R734.2[医药卫生—肿瘤] R532.12[医药卫生—临床医学]