胸腔积液脱落细胞端粒酶逆转录酶mRNA检测及其意义  

Detection of human telomerase reverse transcriptase mRNA expression in exfoliated cell in pleural effusion and its significance

作　　者：仇铁峰[1] 陈萍[2] 丁明[2] 凌夏金 

机构地区：[1]江苏大学附属武进医院呼吸科,江苏武进213002 [2]江苏大学附属医院呼吸科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2008年第5期422-425,共4页Journal of Jiangsu University：Medicine Edition

基　　金：常州市卫生局科技基金资助项目(2006SH002)

摘　　要：目的:通过检测良、恶性胸腔积液脱落细胞端粒酶逆转录酶(hTERT)基因的表达情况,探讨其在良、恶性胸腔积液中的诊断价值。方法:采用逆转录-多聚酶链反应法检测39例恶性胸腔积液、16例良性胸腔积液中hTERT基因的表达情况。结果:恶性胸腔积液中hTERT基因的表达率明显高于良性胸腔积液(P<0.05)。检测hTERT基因表达对恶性胸腔积液的敏感性、特异性和诊断符合率分别为87.18%,81.25%和85.45%。结论:hTERT基因参与了恶性胸腔积液的发生、发展过程。采用RT-PCR法检测胸腔积液中hTERT基因表达有助于临床上良、恶性胸腔积液的鉴别诊断。Objective ： To investigate the diagnostic significance of the detection of the expression of human telomerase reverse transcriptase （hTERT） gene in benign and malignant pleural effusion. Methods： Telomerase hTERT gene expression of exfoliated cell in pleural effusion of 16 cases with benign pleural effusion and 39 cases with malignant pleural effusion were measured by RT-PCR. Results： Telomerase hTERT gene expression of exfoliated cell in malignant pleural effusion was higher than that in benign pleural effusion （ P 〈 0.05 ）. The sensitivity, specificity and diagnostic accordance rate （ % ） of hTERT mRNA was 87. 18%, 81.25% and 85.45%, respectively. Conclusion： Telomerase hTERT gene might involve in pathological process of malignant pleural effusion. The detection of telomerase hTERT gene by RT-PCR is useful to diagnose and distinguish the benign and malignant of pleural effusion.

关 键 词：胸腔积液 端粒酶逆转录酶 逆转录-多聚酶链反应法 

分 类 号：R730.43[医药卫生—肿瘤]