The ORF 113 of Heliocoverpa armigera Single Nucleopolyhedrovirus Encodes a Functional Fibroblast Growth Factor  

作　　者：Xiang LI Chang-yong LIANG Jian-hua SONG Xin-wen CHEN 

机构地区：[1]State Key Laboratory of Virology, Wuhan Institute of Virology, the Chinese Academy of Sciences, Wuhan,430071, China

出　　处：《Virologica Sinica》2008年第5期321-329,共9页中国病毒学（英文版）

基　　金：National Nature Science Foundations of China (30325002,30670077);National Basic Research Priorities Program of China (2003CB1140).

摘　　要：Fibroblast growth factor (FGF) is a key regulator of developmental processes. A FGF homolog (vFGF) is found in all lepidopteran baculoviruses. Autographa californica nucleopolyhedrovirus (AcMNPV) and Bombyx mori NPV (BmNPV) vFGFs are chemotactic factors. Here we analyzed the vfgf of Helicoverpa armigera NPV (HearNPV), a group II NPV. The HearNPV vfgf transcripts were detected from 18 to 96 h post-infection (hpi) of Hz-AM1 cells with HearNPV and encoded a 36 kDa protein, which was secreted into the culture medium. HearNPV vFGF had strong affinity to heparin, a property important for FGF signaling via an FGF receptor. Unlike its AcMNPV homolog, HearNPV vFGF specially chemoattracted Hz-AM1, but not other insect cells such as Sf9 and Se-UCR and not the mammalian cells 293 and HepG2. HearNPV vFGF is also associated with the envelope of BV but is absent in occlusion-derived virus, which coordinated to the chemotatic activity analysis.Fibroblast growth factor （FGF） is a key regulator of developmental processes. A FGF homolog （vFGF） is found in all lepidopteran baculoviruses. Autographa californica nucleopolyhedrovirus （AcMNPV） and Bombyx mori NPV （BmNPV） vFGFs are chemotactic factors. Here we analyzed the vfgf of Helicoverpa armigera NPV （HearNPV）, a group II NPV. The HearNPV vfgf transcripts were detected from 18 to 96 h post-infection （hpi） of Hz-AM1 cells with HearNPV and encoded a 36 kDa protein, which was secreted into the culture medium HearNPV vFGF had strong affinity to heparin, a property important for FGF signaling via an FGF receptor. Unlike its AcMNPV homolog, HearNPV vFGF specially chemoattracted Hz-AM1, but not other insect cells such as Sf9 and Se-UCR and not the mammalian cells 293 and HepG2. HearNPV vFGF is also associated with the envelope of BV but is absent in occlusion-derived virus, which coordinated to the chemotatic activity analysis

关 键 词：Helicoverpa armigera nucleopolyhedrovirus （HearNPV） Fibroblast growth factor （FGF） BACULOVIRUS Open reading frame 113 （ORF 113） 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治] Q516[农业科学—植物保护]