天山雪莲PsbO基因的克隆、原核表达及其烟草中的遗传转化  

Cloning and Construction of Prokaryon and Plant Expression Vector of Saussurea involucrata Kar.et Kir PsbO Gene and Transgenic in Tobacco

作　　者：彭晓明[1] 王重[1] 徐登献[1] 陈尚珂[1] 祝建波[1] 

机构地区：[1]石河子大学生命科学学院农业生物技术重点实验室,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2008年第4期397-401,共5页Journal of Shihezi University(Natural Science)

基　　金：国家863计划(2007AA021401);新疆兵团科技攻关计划(NKBD25DXK01SW)资助项目

摘　　要：从已建立的天山雪莲抗寒cDNA文库中筛选克隆了雪莲的PsbO基因,发现该基因全长1145bp,编码329个氨基酸。通过构建原核表达载体在大肠杆菌中成功获得表达,同时构建了植物表达载体,转化烟草,并获得了部分转基因植株。In the study, we cloned and seclected the PsbO gene from the Saussurea involucrata Kar. et Kir cDNA library and found that it has 1145bp and could code 329AAs in this research. We have constructed the prokaryan expression vector and induced its expression in E. coli successfully. Meanwhile we have constructed the plant expression vector pBinPsbO and acquired some transgenic tobaccos.

关 键 词：OEE1 PSBO 天山雪莲 载体 

分 类 号：Q785[生物学—分子生物学]