不同游泳运动后大鼠下丘脑视上核、室旁核内Fos蛋白表达的变化  

Effects of Different Swimming Styles on the Expression of Fos Protein in PVN and SON of Hypothalamus in Rats

作　　者：王迎春[1] 杨桂姣[1] 张安民[2] 李楠[1] 杨李旺[1] 

机构地区：[1]山西医科大学人体解剖学教研室,山西太原030001 [2]山西财经大学体育系

出　　处：《中国运动医学杂志》2008年第5期563-567,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(30671019)资助

摘　　要：目的:观察游泳运动后大鼠下丘脑内Fos蛋白的定位、分布和时效性表达规律,探讨下丘脑对不同形式运动的调节机制。方法:将55只大鼠随机分为对照组(n=5)和运动组(n=50),运动组又分为持续运动组(n=25)和间歇训练组(n=25)。持续运动组每天游泳2次,每次150min,中间休息120min;间歇训练组每天游泳1次,负重游泳6min后休息4min,反复训练10组。免疫组织化学ABC法检测不同形式运动后即刻(0h)、0.5h、1h、2h、4h大鼠下丘脑内Fos蛋白的定位和分布,并进行图像分析。结果:(1)对照组大鼠下丘脑内Fos阳性神经元少量散在分布;运动组明显增多,在视上核(SON)、室旁核(PVN)等处成簇分布,核团界限清晰。(2)在室旁核小细胞部(pPVN),持续运动组游泳结束后1h Fos阳性神经元数目显著升高达峰值,然后回落;间歇训练组在运动结束后2h达峰值,较运动结束后即刻阳性神经元数显著增多(P<0.05),同一时刻间歇训练组表达显著高于持续运动组(P<0.05);室旁核大细胞部(mPVN)内,持续运动组Fos阳性神经元数在运动结束后持续升高,2h后显著下降,而间歇训练组各时刻阳性神经元数变化无统计学意义。(3)在SON内,大鼠游泳运动结束后4h内Fos阳性神经元数目维持在较高水平,两组内各时间点间无显著性差异(P>0.05)。结论:下丘脑SON和PVN在运动后机体调节中起重要作用,pPVN对不同形式运动性应激反应具有较高灵敏度。Objective To observe the location, distribution and the rhythem of expression of Fos protein after swimming in rats, and investigate the mechanism of hypothalamic regulation to different types of exercise. Methods Fifty-five rats were randomly divided into control group （n=5） and model group （n=50）. Model rats were trained under durative and intermittent swimming. The durative group swam twice a day, and extended 150min each time with a middle brake about 120min, while the intermittent rats been weighted were trained once a day, having a 6min swimming then doing a 4min rest, for 10 times repeatedly. The location and distribution of the Fos stained immunoreactive neurons （Fos＋ neurons） in the hypothalamus were detected at the moments of Oh, 0.5h, 1h, 2h, 4h after swimming by immunohistochemical SABC method and the image were analyzed by imaging analyzing system. Results （1） Several Fos＋ neurons of control group distribute in the hypothalamus dispersively while the swimming groups express significantly higher especially in paraventricular nuclei （PVN） and supraoptic neclei （SON）. （2） In parvicellular part of PVN （pPVN）, the number of Fos＋ neurons rises up to the top at 1h after durative swimming while at 2h after intermittent swimming, moreover, the numbers in the top were significantly higher compared to the moment at Oh （P〈0.05）, and the number in intermittent is numerous than that in durative. In magnocellar part of PVN （mPVN）, for the durative exercise, the Fos ＋ neurons increase gently and then descend at 2h, the number of Fos＋ neuron for intermittent swimming has no statistic change. There is no significant difference for the number of Fos＋ neuron in the 4 hours after swimming-finished in SON （P〉0.05）. Conclusion The SON and PVN in hypothalamus play an important role in physical regulation after exercise; the pPVN is more sensitive to response to different types of exercise stress.

关 键 词：游泳运动 视上核 室旁核 FOS蛋白 大鼠 

分 类 号：R87[医药卫生—运动医学]